Description

n-(2-Aminoethylcarbonyl)-5(6)-Aminofluorescein is a reactive, bifunctional fluorescent labeling reagent that combines the properties of fluorescein with a primary amine-reactive succinimidyl ester group. This compound is critical for creating stable, fluorescent conjugates with proteins, peptides, and other biomolecules, enabling sensitive detection and analysis. It is primarily needed by researchers and manufacturers in the life sciences, diagnostics, and biotechnology sectors for applications in fluorescence microscopy, flow cytometry, immunoassays, and bioanalytical method development.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides via its amine-reactive NHS ester moiety for use in various detection platforms.
• Immunoassay Development: Serving as a key fluorescent tracer or detection reagent in enzyme-linked immunosorbent assays (ELISA), lateral flow assays, and other immunochemical tests.
• Cellular Imaging & Microscopy: Labeling cellular components or specific targets for visualization and tracking in fluorescence microscopy and live-cell imaging studies.
• Flow Cytometry: Preparation of fluorescently-tagged antibodies or probes for the detection and sorting of specific cell populations.
• Bioconjugation & Probe Synthesis: A versatile building block for synthesizing custom fluorescent probes, sensors, and molecular beacons.
• Diagnostic Kit Manufacturing: Integration into commercial diagnostic kits and reagents where a bright, stable fluorescent signal is required.

Basic Information

Product Name	n-(2-Aminoethylcarbonyl)-5(6)-Aminofluorescein
CAS No.	73857-22-4
Molecular Formula	C25H19N2O7
Molecular Weight	459.43 g/mol
Synonyms	5(6)-FAM, SE; 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester; 6-FAM, SE; 5(6)-Carboxyfluorescein succinimidyl ester; FAM, SE isomer mixture; Fluorescein-5(6)-isothiocyanate isomer I; 5(6)-FAM NHS ester; 6-Carboxyfluorescein N-succinimidyl ester
EINECS	Contact for details

Quality Control

Our n-(2-Aminoethylcarbonyl)-5(6)-Aminofluorescein is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure consistency and performance for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥95.0% (Isomer mixture)
Extinction Coefficient	~68,000 cm⁻¹M⁻¹ at 492 nm (pH 9)
Water Content (KF)	≤5.0%
Solubility	Soluble in DMF, DMSO; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.