Description

n-(7-Nitrobenzofurazan-4-Yl)Phallacidin is a specialized fluorescent-labeled derivative of the potent toxin phalloidin, designed for advanced research applications. This compound is critical for high-sensitivity detection and visualization of filamentous actin (F-actin) in cellular and biochemical studies. It is primarily utilized by researchers and scientists in the fields of cell biology, pharmacology, and toxicology. The product is supplied with a focus on high purity and batch-to-batch consistency to ensure reliable experimental results.

Application

• F-Actin Staining and Visualization: As a high-affinity, fluorescent probe for labeling and imaging actin filaments in fixed cells using fluorescence microscopy.
• Cytoskeleton Research: For studying the dynamics, organization, and role of the actin cytoskeleton in cellular processes like motility, division, and signaling.
• Toxicology Studies: Used as a tool to investigate the mechanism of action of amatoxins and phallotoxins, and their effects on cell structure.
• Pharmaceutical Screening: Employed in assays to identify compounds that affect actin polymerization or depolymerization for drug discovery.
• Biochemical Assays: For quantitative analysis of F-actin content in solution-based or in vitro binding studies.
• Neuroscience Research: Visualization of actin structures in neurons, crucial for understanding synaptic plasticity and morphology.

Basic Information

Product Name	n-(7-Nitrobenzofurazan-4-Yl)Phallacidin
CAS No.	73413-78-2
Molecular Formula	C44H56N8O14S
Molecular Weight	953.04 g/mol
Synonyms	NBD-Phallacidin; 4-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl))Phallacidin; 7-Nitrobenz-2-oxa-1,3-diazol-4-yl Phallacidin; NBD-phallacidin, fluorescent probe; Phallacidin, NBD-labeled; Fluorescent phallacidin derivative; Actin stain NBD-phallacidin; NBD-conjugated phallacidin
EINECS	Contact for details

Quality Control

Our n-(7-Nitrobenzofurazan-4-Yl)Phallacidin is produced and handled under strict quality protocols to ensure it meets the exacting standards required for life science research. Each batch is subjected to comprehensive analytical testing, including purity assessment by HPLC and spectroscopic identification. A Certificate of Analysis (COA) detailing batch-specific results is provided to guarantee traceability and performance for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be kept in a dry environment. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, methanol
Fluorescence Properties	Excitation ~465 nm, Emission ~535 nm
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.