Description

N,1-Bis(Trimethylsilyl)-4-(Trimethylsilyl)Oxy-3-[2-O,3-O,5-o-Tris(Trimethylsilyl)-β-D-Ribofuranosyl]-1H-Pyrazole-5-Carboxamide is a highly specialized, multi-silylated nucleoside derivative, serving as a critical protected intermediate in advanced organic synthesis. Its primary value lies in providing robust protection for reactive hydroxyl and amide groups, enabling complex multi-step reactions with high selectivity and yield. This compound is essential for pharmaceutical R&D and fine chemical manufacturers focused on developing novel antiviral agents, modified oligonucleotides, and other bioactive molecules.

Application

• Nucleoside Analogue Synthesis: A key protected intermediate in the synthesis of modified ribonucleosides and their analogues for antiviral and anticancer research.
• Oligonucleotide Chemistry: Used in the preparation of silyl-protected phosphoramidite monomers for solid-phase oligonucleotide synthesis.
• Pharmaceutical Intermediates: Serves as a building block for the development of prodrugs and active pharmaceutical ingredients (APIs) targeting viral polymerases.
• Glycosylation Reactions: Acts as a glycosyl donor or acceptor in stereoselective glycosylation steps due to its fully silylated ribose moiety.
• Chemical Biology Probes: Utilized in the creation of labeled or modified nucleosides for studying enzymatic mechanisms and nucleic acid interactions.
• Process Chemistry Development: Employed in scaling up synthetic routes for nucleotide-based therapeutics, where its protecting groups offer stability during purification.

Basic Information

Item	Details
Product Name	N,1-Bis(Trimethylsilyl)-4-(Trimethylsilyl)Oxy-3-[2-O,3-O,5-o-Tris(Trimethylsilyl)-β-D-Ribofuranosyl]-1H-Pyrazole-5-Carboxamide
CAS No.	72360-96-4
Molecular Formula	C27H61N3O6Si6
Molecular Weight	684.44 g/mol
Synonyms	1H-Pyrazole-5-carboxamide, N,1-bis(trimethylsilyl)-4-(trimethylsilyl)oxy-3-[2,3,5-tris-O-(trimethylsilyl)-β-D-ribofuranosyl]-; 3-β-D-Ribofuranosyl-1H-pyrazole-5-carboxamide, N,1,4-tris-O-(trimethylsilyl) derivative; Fully silylated ribofuranosyl pyrazolecarboxamide; TMS-protected ribonucleoside intermediate; 72360-96-4; 5-Carboxamido-1H-pyrazole riboside per-O-TMS derivative
EINECS	Contact for details

Quality Control

Every batch of this high-value intermediate is produced and analyzed under strict quality management systems. We employ advanced analytical techniques including HPLC, NMR, and MS to verify identity, purity, and the integrity of the silyl protecting groups. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to ensure it meets the exacting standards required for GMP-grade pharmaceutical synthesis.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its highly volatile and moisture-sensitive nature (hygroscopic), the container must be kept tightly sealed under an inert atmosphere (argon or nitrogen) after opening to prevent degradation and desilylation. Keep away from strong bases and incompatible materials.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 97.0% (by NMR)
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.