Description

Fmoc-Pro-Onp CAS NO 71989-32-7 is a specialized amino acid derivative where the proline residue is protected with a 9-fluorenylmethoxycarbonyl (Fmoc) group and activated with an o-nitrophenyl (Onp) ester. This compound is a critical building block in modern solid-phase peptide synthesis (SPPS), offering high reactivity and selectivity for efficient peptide chain elongation. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of novel therapeutic peptides, peptide hormones, and biochemical probes.

Application

• Solid-Phase Peptide Synthesis (SPPS): As an activated ester for the efficient incorporation of proline into growing peptide chains under mild conditions.
• Pharmaceutical Research & Development: For the synthesis of peptide-based drug candidates, including enzyme inhibitors and receptor agonists/antagonists.
• Bioconjugation: Used to attach proline-containing peptide sequences to other molecules, surfaces, or solid supports for creating labeled probes.
• Combinatorial Chemistry: A key reagent in libraries designed for high-throughput screening of bioactive peptides.
• Academic Research: For studying peptide structure-activity relationships (SAR) and protein-protein interactions.
• Production of Custom Peptides: Employed by CROs and CDMOs for the reliable and scalable manufacture of complex peptides for clients.

Basic Information

Product Name	Fmoc-Pro-Onp
CAS No.	71989-32-7
Molecular Formula	C25H20N2O6
Molecular Weight	444.44 g/mol
Synonyms	Fmoc-Proline ONP ester; Fmoc-L-Proline 4-nitrophenyl ester; N-(9-Fluorenylmethoxycarbonyl)-L-proline 4-nitrophenyl ester; 1-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-proline 4-nitrophenyl ester; Fmoc-Pro-ONp; Fmoc-Pro-OPnp; Fmoc-Proline o-nitrophenyl ester; Nα-Fmoc-L-proline p-nitrophenyl ester
EINECS	Contact for details

Quality Control

Every batch of Fmoc-Pro-Onp is manufactured and tested under strict quality management systems to ensure consistency and reliability for sensitive synthetic applications. Our products undergo rigorous quality testing, including HPLC for purity and identity confirmation, to ensure compliance with industry standards. Certificates of Analysis (COA) detailing batch-specific results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent hydrolysis of the active ester. For long-term storage, consider desiccant and inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Specific Rotation	Contact for details
Water Content (KF)	≤ 0.5%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.