Description

p-Tolyltetrazolium Red is a specialized tetrazolium salt widely used as a vital stain and redox indicator in biochemical and microbiological research. Its core value lies in its ability to form a vivid, insoluble formazan dye upon reduction, providing a clear colorimetric signal for detecting cellular metabolic activity. This makes it an essential reagent for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life science sectors, particularly for applications like cell viability assays and microbiological testing.

Application

• Cell Viability and Cytotoxicity Assays: Used to quantify the metabolic activity of live cells in culture, serving as a key endpoint in drug screening and toxicology studies.
• Microbiological Testing: Employed as a redox indicator to detect microbial growth and contamination in sterility testing and environmental monitoring.
• Enzyme Activity Studies: Acts as an electron acceptor in dehydrogenase enzyme assays, facilitating the spectrophotometric measurement of enzymatic rates.
• Histological Staining: Utilized in tissue sections to localize areas of metabolic activity or ischemic damage.
• Seed Viability Testing: Applied in agricultural research to assess seed germination potential and vigor based on respiratory activity.
• Biomedical Research: Supports studies in apoptosis, proliferation, and oxidative stress within cellular models.

Basic Information

Product Name	p-Tolyltetrazolium Red
CAS No.	71658-33-8
Molecular Formula	C19H15N5O
Molecular Weight	329.36 g/mol
Synonyms	2-(4-Methylphenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride; Tetrazolium Red; TTC Red; p-Tolyl Tetrazolium Red; INT; Iodonitrotetrazolium chloride analog; Red Tetrazolium; 4-Methyl-2-(4-nitrophenyl)-3,5-diphenyl-2H-tetrazolium chloride
EINECS	Contact for details

Quality Control

Every batch of p-Tolyltetrazolium Red is manufactured and tested under a strict quality management system to ensure high purity and consistent performance for sensitive research applications. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed after each use to prevent absorption of atmospheric moisture, which can affect stability and performance.

Specification

Item	Specification
Appearance	Red to dark red powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in water, ethanol, DMSO
Biological Activity Test	Passes performance test in standard cell viability assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.