Description

3'-o-Methylfluorescein CAS NO 70672-05-8 is a chemically modified derivative of fluorescein, a cornerstone fluorophore in life science research. This compound is valued for its tailored photophysical properties, offering researchers a versatile tool for labeling, detection, and assay development. It is primarily utilized by professionals in the pharmaceutical, biotechnology, and diagnostic industries for applications requiring high-sensitivity fluorescence.

Application

• Fluorescent Labeling Agent: Used for covalent conjugation to biomolecules (e.g., proteins, peptides, antibodies) for fluorescence microscopy and flow cytometry.
• Enzyme Substrate & Probe Development: Serves as a core structure for synthesizing substrates to detect enzymatic activity, particularly for proteases and esterases.
• Diagnostic Assay Component: Incorporated into immunoassays and molecular diagnostic kits as a detection tracer.
• Biochemical Research: Employed in fluorescence polarization (FP), fluorescence resonance energy transfer (FRET), and other advanced biophysical studies.
• Chemical Sensor Synthesis: Acts as a signaling unit in the design of chemosensors for detecting ions or small molecules.
• Pharmaceutical Analysis: Used as a derivatization agent or reference standard in analytical method development (HPLC, LC-MS).

Basic Information

Product Name	3'-o-Methylfluorescein
CAS No.	70672-05-8
Molecular Formula	C22H16O5
Molecular Weight	360.36 g/mol
Synonyms	3'-O-Methylfluorescein; 3'-Methoxyfluorescein; 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic Acid Methyl Ether; 9-(2-Carboxyphenyl)-6-hydroxy-3-methoxyxanthen-3-one; Fluorescein 3'-Methyl Ether; 3'-OMe-Fluorescein
EINECS	Contact for details

Quality Control

Our 3'-o-Methylfluorescein is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and loss on drying. We provide a detailed Certificate of Analysis (COA) with every shipment to ensure traceability and compliance with your research or production specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Loss on Drying	≤ 1.0 %
Residue on Ignition	≤ 0.5 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.