Description

Nbd-5-Acylcholine CAS NO 70214-86-7 is a specialized fluorescent-labeled choline derivative, designed for advanced biochemical research and diagnostic applications. This compound serves as a critical tool for the sensitive detection and study of cholinergic systems, offering high specificity and signal-to-noise ratios. It is primarily utilized by researchers and developers in the fields of neurochemistry, molecular biology, and pharmaceutical development for probing enzyme activity, receptor binding, and cellular transport mechanisms.

Application

• Biochemical Research Probe: Used as a fluorescent substrate for studying acetylcholinesterase and choline acetyltransferase activity in vitro.
• Neuropharmacology: Enables visualization and quantification of choline uptake and metabolism in neuronal cell cultures and tissue samples.
• Diagnostic Assay Development: Serves as a key component in developing high-throughput screening (HTS) assays for drug discovery targeting the cholinergic pathway.
• Cellular Imaging: Facilitates real-time, fluorescent imaging of cholinergic synapse function and dynamics.
• Enzyme Kinetics Studies: Provides a sensitive means to measure kinetic parameters of enzymes involved in acetylcholine synthesis and degradation.

Basic Information

Product Name	Nbd-5-Acylcholine
CAS No.	70214-86-7
Molecular Formula	C₁₈H₂₈N₄O₄⁺ (typically as a salt, e.g., iodide)
Molecular Weight	~364.44 g/mol (free base)
Synonyms	NBD-5-ACh; NBD-Labeled Acetylcholine; 4-(N-(2-(Acetyloxy)ethyl)-N-methylamino)-7-nitro-2,1,3-benzoxadiazole; 7-Nitrobenz-2-oxa-1,3-diazol-4-yl-labeled Acetylcholine; Fluorescent Acetylcholine Analog; Choline, 2-[(7-nitro-4-benzofurazanyl)amino]ethyl ester, acetate (salt); NBD-C2-Choline
EINECS	Contact for details

Quality Control

Every batch of Nbd-5-Acylcholine is manufactured and analyzed under strict quality protocols to ensure consistency and reliability for research purposes. Our products undergo rigorous quality testing, including purity verification by HPLC and structural confirmation, to ensure compliance with industry standards. Certificates of Analysis (COA) detailing batch-specific results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. For long-term storage, consider under an inert atmosphere. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, methanol; sparingly soluble in water
Fluorescence Properties	Ex/Em ~465/535 nm (solvent dependent)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.