Description

Fluorescein Dibutyrate is a lipophilic ester derivative of fluorescein, widely used as a critical substrate in enzymatic assays. Its primary value lies in its role as a non-fluorescent precursor that is hydrolyzed by specific enzymes, such as esterases and lipases, to release the highly fluorescent fluorescein molecule. This property makes it indispensable for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require sensitive and quantitative detection methods for enzyme activity and inhibition studies.

Application

• Enzyme Activity Assays: Key substrate for fluorometric detection of esterase and lipase activity in biochemical research and high-throughput screening (HTS).
• Diagnostic Reagent Manufacturing: Used in the production of diagnostic kits for liver function, pancreatic function, and other clinical tests measuring esterase levels.
• Cell Biology & Microscopy: Employed as a cell-permeant probe for intracellular esterase activity, serving as a viability indicator and a tool for fluorescence-activated cell sorting (FACS).
• Pharmaceutical Development: Utilized in drug discovery to screen for potential inhibitors or activators of carboxylesterase enzymes, which are involved in drug metabolism.
• Food & Industrial Enzyme Analysis: Applied in quality control and research to measure lipase/esterase activity in food processing, detergents, and other industrial enzyme preparations.

Basic Information

Product Name	Fluorescein Dibutyrate
CAS No.	7298-65-9
Molecular Formula	C28H26O7
Molecular Weight	474.50 g/mol
Synonyms	Fluorescein Di-n-butyrate; 3',6'-Bis(butyryloxy)fluoran; 3',6'-Dibutyrylfluorescein; Dibutyryl Fluorescein; Fluorescein Dibutanoate; FD; FDB; NSC 404795
EINECS	Contact for details

Quality Control

Our Fluorescein Dibutyrate is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We support compliance with cGMP and ISO 9001 standards for our manufacturing processes, ensuring reliability for critical industrial and pharmaceutical uses.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry, cool place. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm
Fluorescence Background	Low (specified in COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.