Description

5-Bromo-6-Chloro-3-Indoxyl Nonanoate is a specialized chromogenic enzyme substrate used in advanced biochemical and diagnostic applications. This compound is critical for the sensitive detection and quantification of specific enzymatic activities, particularly esterases and lipases, due to its ability to produce an insoluble, colored precipitate upon cleavage. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical development laboratories. The high specificity and performance of this substrate make it an essential reagent for histochemical staining, blotting techniques, and high-throughput screening assays.

Application

• Histochemical Staining Agent: Used as a substrate for localizing esterase and lipase activity in tissue sections, producing a vivid, insoluble indigo dye for microscopic analysis.
• Diagnostic Reagent Manufacturing: A key component in the formulation of clinical diagnostic kits for detecting specific enzyme markers related to liver function, pancreatic health, and other metabolic conditions.
• Biochemical Research & Assay Development: Employed in enzymatic assays within life science research to study enzyme kinetics, inhibition, and activity profiling under various experimental conditions.
• Microbiology & Cell Biology: Utilized for staining bacterial colonies expressing recombinant enzymes or for detecting enzymatic activity in live-cell imaging and flow cytometry applications.
• Pharmaceutical Screening: Serves as a critical tool in high-throughput screening (HTS) platforms within drug discovery to identify potential modulators of target esterase enzymes.
• Blotting Techniques: Applied in Western, Northern, and Southern blot protocols as a chromogenic substrate for enzyme-linked detection systems, providing a colorimetric readout.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indoxyl Nonanoate
CAS No.	682802-80-8
Molecular Formula	C17H21BrClNO3
Molecular Weight	402.71 g/mol
Synonyms	5-Bromo-6-chloro-3-indolyl nonanoate; BCIP Nonanoate; 3-Indoxyl Nonanoate, 5-Bromo-6-Chloro derivative; 5-Br-6-Cl-3-Indoxyl Nonanoate; Nonanoic acid 5-bromo-6-chloro-1H-indol-3-yl ester; Chromogenic esterase substrate; Indoxyl ester substrate; BCIP-Nonanoate
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indoxyl Nonanoate is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and the absence of interfering impurities. We provide a comprehensive Certificate of Analysis (COA) with each shipment, detailing all relevant specifications. Our quality system is designed to meet the rigorous standards required for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccation and under an inert atmosphere to maintain optimal stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in alcohols
Functional Performance	Yields intense blue precipitate upon enzymatic cleavage

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.