Description

Atto 590 Nhs Ester* is a high-performance, amine-reactive fluorescent labeling reagent designed for the covalent conjugation of biomolecules. Its core value lies in providing a bright, photostable far-red fluorescent tag with excellent water solubility, enabling sensitive detection and imaging applications. This product is essential for researchers and manufacturers in the fields of bioconjugation, flow cytometry, fluorescence microscopy, and nucleic acid labeling who require reliable and consistent labeling efficiency.

Application

• Protein and Antibody Labeling: Conjugation to lysine residues for creating fluorescent probes for immunoassays (e.g., ELISA, Western blotting) and cellular imaging.
• Oligonucleotide and Nucleic Acid Probes: Labeling of amino-modified DNA or RNA for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Flow Cytometry & Cell Sorting: Preparation of fluorescently-tagged antibodies or other ligands for high-throughput cell surface marker analysis and population sorting.
• Fluorescence Microscopy: Creation of specific cellular probes for confocal and super-resolution microscopy, benefiting from its far-red emission which minimizes autofluorescence.
• Biomaterial and Nanoparticle Functionalization: Covalent attachment to amine-coated surfaces, microspheres, or nanoparticles for diagnostic and sensing platforms.
• Drug Delivery & Targeting Studies: Fluorescent labeling of peptides, proteins, or drug carriers to track distribution, uptake, and pharmacokinetics in vitro and in vivo.

Basic Information

Product Name	Atto 590 Nhs Ester*
CAS No.	670269-33-7
Molecular Formula	C38H41N4O8S2+ (Counter ion typically Trifluoroacetate)
Molecular Weight	~745.9 g/mol (for cation)
Synonyms	ATTO 590 NHS ester; ATTO 590 N-hydroxysuccinimide ester; 5-Isomer ATTO 590 SE; ATTO 590 Succinimidyl Ester; N-Succinimidyl ester of ATTO 590; Fluorescent dye NHS ester (ATTO 590); Reactive dye for amine labeling (far-red)
EINECS	Contact for details

Quality Control

Every batch of Atto 590 Nhs Ester* is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure high labeling efficiency and batch-to-batch consistency through rigorous HPLC analysis for purity and functional validation. Certificates of Analysis (COA) detailing spectral properties, purity, and functional performance are provided with each shipment to support your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and strictly light-sensitive (protect from all light exposure). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 90% (Dye content)
Absorption Maximum (λmax)	~590 nm
Emission Maximum	~624 nm
Extinction Coefficient (ε)	~120,000 L·mol-1·cm-1
Solubility	DMSO, DMF, acetonitrile, water (sufficient for labeling reactions)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.