Description

Bdp 581/591 Nhs Ester CAS NO 654651-21-5 is a high-purity, functionalized fluorescent dye derivative designed for advanced bioconjugation and labeling applications. This compound is critical for researchers and manufacturers requiring precise, covalent attachment of fluorophores to biomolecules like proteins, antibodies, and oligonucleotides. It is primarily used in the development of diagnostic assays, flow cytometry reagents, and advanced microscopy probes within the pharmaceutical, biotechnology, and life science research sectors.

Application

• Bioconjugation Reagent: Covalent labeling of primary amines (-NH2) on proteins, peptides, and antibodies for fluorescence-activated cell sorting (FACS) and immunofluorescence.
• Diagnostic Assay Development: Synthesis of fluorescent probes and tracers for ELISA, lateral flow assays, and other clinical diagnostic platforms.
• Bioimaging & Microscopy: Preparation of specific fluorescent tags for confocal microscopy, super-resolution imaging, and in vivo imaging studies.
• Oligonucleotide Labeling: Fluorescent labeling of DNA and RNA probes for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Chemical Biology Research: Tool for studying protein-protein interactions, localization, and dynamics within live cells.
• Material Science: Functionalization of nanoparticles and polymers for creating smart, fluorescent materials.

Basic Information

Product Name	Bdp 581/591 Nhs Ester
CAS No.	654651-21-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	BDP 581/591 NHS Ester; BODIPY® 581/591 NHS Ester; 4,4-Difluoro-5-(4-Methoxyphenyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionic Acid, Succinimidyl Ester; BODIPY® FL NHS Ester (variant); BDP FL NHS Ester; Boron Dipyrromethene NHS Ester; Fluorescent Dye NHS Ester
EINECS	Contact for details

Quality Control

Our Bdp 581/591 Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95%
Absorption Max (λmax)	~581 nm
Emission Max (λem)	~591 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.