Description

Atto 425 is a high-purity fluorescent dye belonging to the Atto series, known for its excellent photostability and brightness. This compound is essential for advanced research and diagnostic applications requiring precise and reliable fluorescent labeling. It is primarily used by professionals in the life sciences, analytical chemistry, and materials science sectors for applications such as fluorescence microscopy, flow cytometry, and single-molecule detection.

Application

• Fluorescence Microscopy: Used as a robust fluorescent label for cellular and subcellular imaging due to its high photostability.
• Flow Cytometry: Employed in the labeling of antibodies, proteins, and other biomolecules for high-throughput cell analysis.
• Single-Molecule Detection (SMD): Ideal for techniques requiring high signal-to-noise ratios and minimal photobleaching.
• Bioconjugation: Serves as a reactive dye for covalent attachment to amines, thiols, or other functional groups on biomolecules.
• Nucleic Acid Labeling: Used in fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Sensor Development: Incorporated into chemical and biological sensors for detection and diagnostic purposes.

Basic Information

Product Name	Atto 425
CAS No.	652966-03-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	ATTO 425; ATTO-425; 652966-03-5; Atto425; Atto 425 dye; Fluorescent dye Atto 425
EINECS	Contact for details

Quality Control

Our Atto 425 is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including purity assessment by HPLC and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to guarantee compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage, consider keeping the product desiccated under an inert atmosphere to maintain optimal stability and performance.

Specification

Item	Specification
Appearance	Dark blue to purple solid or solution
Identification (HPLC/UV-Vis)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Extinction Coefficient	Contact for details
Fluorescence Quantum Yield	Contact for details
Solubility	Soluble in DMSO, DMF, water (depending on functionalization)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.