Description

Atto 590 is a high-performance, far-red fluorescent dye belonging to the Atto series, known for its exceptional photostability and brightness. This compound is critical for advanced fluorescence-based detection and imaging applications where signal integrity and low background are paramount. It is primarily utilized by researchers and manufacturers in the life sciences, diagnostics, and analytical chemistry sectors for demanding applications such as single-molecule detection, super-resolution microscopy, and flow cytometry.

Application

• Single-Molecule Fluorescence Spectroscopy (SMFS): Ideal for tracking and studying individual biomolecules due to its high photon output and stability.
• Super-Resolution Microscopy (STED, PALM/STORM): Enables imaging beyond the diffraction limit, providing nanoscale spatial resolution in biological samples.
• Flow Cytometry & Cell Sorting: Used for labeling antibodies, proteins, or nucleic acids to detect and isolate specific cell populations.
• Fluorescence In Situ Hybridization (FISH): Serves as a bright and stable label for nucleic acid probes in genetic testing and cytogenetics.
• Biosensor & Diagnostic Assay Development: Incorporated into probes and sensors for highly sensitive detection of biomarkers.
• Polymerase Chain Reaction (PCR) & qPCR: Functions as a reporter dye in probe-based quantitative PCR applications.
• Protein & Nucleic Acid Labeling: Conjugated to biomolecules for tracking interactions, localization, and quantification in complex mixtures.
• Materials Science Research: Used in developing fluorescent nanomaterials and studying polymer dynamics.

Basic Information

Product Name	Atto 590
CAS No.	639065-13-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	ATTO 590; ATTO-590; ATTO590; Atto 590 dye; 639065-13-7; Far-red fluorescent dye ATTO 590; ATTO™ 590
EINECS	Contact for details

Quality Control

Our Atto 590 is produced under stringent quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance/fluorescence verification, and functional performance testing. A detailed Certificate of Analysis (COA) documenting identity, purity, and spectral properties is provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a temperature between 2°C and 8°C for long-term stability. The product is light-sensitive (store away from light). Allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Dark red to purple solid or solution
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~590 nm
Emission Maximum (λem)	~615 nm
Extinction Coefficient (ε)	≥ 120,000 L·mol⁻¹·cm⁻¹
Fluorescence Quantum Yield (φF)	≥ 0.8 (reference dependent)
Solubility	Soluble in DMSO, DMF, water (as salt form)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.