Description

Cyanine 3 Maleimide is a near-infrared fluorescent dye functionalized with a maleimide group for site-specific bioconjugation. This compound is essential for creating highly stable, fluorescently labeled probes for advanced bioanalytical and diagnostic applications. It is primarily used by researchers and manufacturers in the fields of biopharmaceutical development, in vitro diagnostics, and life science research who require precise and sensitive detection methods.

Application

• Bioconjugation and Probe Development: Site-specific labeling of proteins, antibodies, and peptides via cysteine thiol groups for fluorescence imaging and detection.
• In Vitro Diagnostics (IVD): Manufacturing of fluorescent reagents and kits for immunoassays, flow cytometry, and lateral flow assays.
• Biopharmaceutical R&D: Tracking and analysis of biomolecules in drug discovery, including target engagement and pharmacokinetic studies.
• Molecular Imaging: Development of contrast agents for fluorescence microscopy and small animal imaging in preclinical research.
• Nucleic Acid Labeling: Conjugation to modified oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Material Science: Functionalization of nanoparticles and polymers to create smart materials with fluorescent properties.

Basic Information

Product Name	Cyanine 3 Maleimide
CAS No.	616207-80-8
Molecular Formula	C33H38N3O3+
Molecular Weight	524.67 g/mol
Synonyms	Cy3 Maleimide; Cy™3 Maleimide; Sulfo-Cyanine3 Maleimide (derivative); 1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)-1-oxopentyl]-3,3-dimethyl-1-(3-sulfopropyl)-, inner salt; Cyanine 3 C2 Maleimide; Cy3® Maleimide; 2-[(1E,3E)-5-[(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)]-1-(3-sulfonatopropyl)-3,3-dimethylindolin-2-ylidene]pent-1-en-1-yl]-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate (isomeric form)
EINECS	Contact for details

Quality Control

Our Cyanine 3 Maleimide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	548 - 552 nm
Emission (λmax)	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm-1M-1
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.