Description

Eosin-5-Iodoacetamide is a fluorescent thiol-reactive probe, widely recognized for its utility in protein labeling and biochemical research. Its core value lies in its ability to form stable thioether bonds with cysteine residues, enabling the sensitive detection and analysis of proteins via fluorescence spectroscopy and microscopy. This reagent is essential for researchers and professionals in the fields of proteomics, molecular biology, and diagnostic assay development who require reliable and specific protein conjugation.

Application

• Protein Labeling and Conjugation: Specifically modifies cysteine residues in proteins for fluorescence-based studies.
• Biomolecular Assay Development: Used as a key component in developing fluorescent immunoassays and diagnostic kits.
• Gel Electrophoresis and Western Blotting: Serves as a fluorescent tag for pre-staining proteins to monitor purification or transfer efficiency.
• Cell Biology and Imaging: Enables the tracking and localization of thiol-containing biomolecules within live or fixed cells.
• Proteomics Research: Facilitates the study of protein structure, function, and protein-protein interactions.
• Bioconjugation for Drug Delivery: Acts as a linker for attaching therapeutic agents or targeting moieties to carrier proteins.

Basic Information

Product Name	Eosin-5-Iodoacetamide
CAS No.	69414-31-9
Molecular Formula	C20H8Br2I2N2O5
Molecular Weight	785.90 g/mol
Synonyms	5-((2-(Iodoacetamido)ethyl)amino)eosin; Eosin 5-iodoacetamide; 5-IAE; 5-((2-(Iodoacetamido)ethyl)amino)eosin; 2',4',5',7'-Tetrabromo-3',6'-dihydroxy-5-((2-(iodoacetamido)ethyl)amino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
EINECS	Contact for details

Quality Control

Our Eosin-5-Iodoacetamide is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as identity, purity, and impurity profiles. We adhere to relevant industry standards to support your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to moisture and light.

Specification

Item	Specification
Appearance	Red to reddish-brown powder
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.