Description

n-Succinimidyl 4-Nitrophenylacetate is a highly reactive, bifunctional N-hydroxysuccinimide (NHS) ester derivative used as a versatile crosslinking and bioconjugation reagent. Its core value lies in its ability to efficiently form stable amide bonds with primary amines under mild aqueous conditions, making it indispensable for modifying biomolecules. This compound is primarily needed by researchers and manufacturers in the fields of biochemistry, proteomics, and pharmaceutical development for labeling proteins, peptides, and other amine-containing compounds.

Application

• Protein and Antibody Labeling: Conjugation of fluorescent dyes, biotin, or other probes to primary amine groups (e.g., lysine residues) for detection and imaging studies.
• Peptide Synthesis and Modification: Activation of carboxylic acid groups for subsequent coupling reactions in solid-phase peptide synthesis (SPPS) or solution-phase modifications.
• Immobilization Chemistry: Functionalization of sensor chips, microarray surfaces, or chromatography resins with active esters for covalent attachment of biomolecules.
• Crosslinking Agent: Creation of covalent linkages between different proteins or between proteins and other polymers in material science and drug delivery system development.
• Preparation of Active Esters: Serving as a precursor or intermediate for synthesizing more complex or specialized NHS-ester reagents.
• Bioconjugation for ADC Development: Facilitating the linker chemistry in the synthesis of Antibody-Drug Conjugates (ADCs) and other targeted therapeutics.

Basic Information

Product Name	n-Succinimidyl 4-Nitrophenylacetate
CAS No.	68123-33-1
Molecular Formula	C12H10N2O6
Molecular Weight	278.22 g/mol
Synonyms	4-Nitrophenylacetic Acid N-Succinimidyl Ester; N-Succinimidyl 4-Nitrophenyl Acetate; Suc-ONp; NHS-4-Nitrophenylacetate; 4-Nitrophenyl Acetate N-Hydroxysuccinimide Ester; N-(4-Nitrophenylacetoxy)succinimide; 4-NPA-OSu; 4-Nitrophenylacetic Acid N-Hydroxysuccinimide Ester
EINECS	Contact for details

Quality Control

Our n-Succinimidyl 4-Nitrophenylacetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) providing detailed specifications for purity (HPLC), identity (IR, NMR), and moisture content are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. Due to its hygroscopic and moisture-sensitive nature, the container should be sealed under an inert atmosphere (e.g., nitrogen or argon) after each use to maintain stability and prevent hydrolysis.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	158 - 162 °C
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.