Description

Ethidium Diazide is a specialized photoactivatable chemical crosslinker derived from the classic nucleic acid stain, ethidium bromide. This compound is critical for researchers requiring precise, light-triggered covalent binding to DNA or RNA, enabling advanced structural and interaction studies. It is primarily utilized in molecular biology and biochemistry laboratories for applications such as nucleic acid footprinting, structural probing, and the analysis of protein-nucleic acid complexes.

Application

• Nucleic Acid Footprinting & Structural Probing: Used to covalently crosslink with DNA or RNA upon photoactivation, mapping protein binding sites and determining nucleic acid secondary structure.
• Study of Protein-Nucleic Acid Interactions: Facilitates the irreversible trapping of protein-DNA or protein-RNA complexes for subsequent isolation and analysis.
• Biophysical Research: Employed in advanced biophysical techniques to investigate the dynamics and architecture of nucleic acids and their complexes.
• Development of Diagnostic Assays: Serves as a key reagent in the development of novel crosslinking-based assays for detecting specific nucleic acid sequences or structures.
• Photochemical Crosslinking Reagent: Acts as a vital tool in photochemistry research for studying light-induced reactions with biomolecules.

Basic Information

Product Name	Ethidium Diazide
CAS No.	67620-23-9
Molecular Formula	C23H20N6O2
Molecular Weight	412.45 g/mol
Synonyms	Ethidium Homodiazirine; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium Diazide; Photoactivatable Ethidium Bromide; Ethidium Monoazide Analog; Phenanthridinium Diazide; DNA Photo-Crosslinker; RNA Crosslinking Reagent
EINECS	Contact for details

Quality Control

Our Ethidium Diazide is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics. We adhere to relevant quality management standards to guarantee the reliability of this critical biochemical reagent.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent degradation due to its light-sensitive and easily oxidized nature. Allow the container to reach room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Red to brown solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Photoactivation Efficiency	Functional by gel shift assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.