Description

4-Hydroperoxy-Cyclophosphamide is a key oxidative metabolite and synthetic intermediate of the alkylating agent cyclophosphamide. This compound matters for researchers and manufacturers as it provides a direct, stable precursor for studying metabolic activation pathways and developing related pharmaceutical agents. It is primarily needed by the pharmaceutical, biotechnology, and life science research sectors for advanced drug metabolism studies, prodrug development, and as a critical reference standard in analytical chemistry.

Application

• Pharmaceutical Research & Development: As a crucial oxidative metabolite for studying the bioactivation and pharmacokinetics of cyclophosphamide.
• Reference Standard: Used in analytical laboratories for HPLC, LC-MS, and NMR method development and validation to quantify cyclophosphamide and its metabolites.
• Biochemical Research: Investigation of DNA alkylation mechanisms and cellular response to activated chemotherapeutic agents in oncology research.
• Prodrug Synthesis: Serves as a synthetic intermediate in the development and preparation of novel prodrug candidates and targeted therapies.
• Toxicology Studies: Employed to understand the metabolic pathways and potential toxic side effects associated with cyclophosphamide therapy.

Basic Information

Product Name	4-Hydroperoxy-Cyclophosphamide
CAS No.	67292-61-9
Molecular Formula	C7H15Cl2N2O3P
Molecular Weight	277.09 g/mol
Synonyms	4-Hydroperoxycyclophosphamide; 4-HP-CPA; Cyclophosphamide Monoperoxide; 2-[Bis(2-chloroethyl)amino]tetrahydro-2H-1,3,2-oxazaphosphorine 4-oxide 4-hydroperoxide; NSC 181815; Peroxycyclophosphamide; 4-Hydroperoxy Cyclophosphamide
EINECS	Contact for details

Quality Control

Our 4-Hydroperoxy-Cyclophosphamide is produced and handled under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and development. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting batch-specific results.

Storage

Preserve in a tightly closed container, protected from light. Store under an inert atmosphere (e.g., nitrogen or argon) at recommended cold chain conditions of 2-8°C or as specified on the label or COA. This product is light-sensitive and easily oxidized; prolonged exposure to air, light, or elevated temperatures should be avoided to maintain stability and potency.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	Individual impurity ≤ 1.0%
Total Impurities	≤ 5.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.