Description

Hippuryl n-Hydroxysuccinimide Ester is a specialized, high-purity active ester derivative used as a critical reagent in peptide synthesis and bioconjugation chemistry. Its primary value lies in its reactivity as an acylating agent, enabling the efficient and selective formation of amide bonds under mild conditions, which is essential for constructing complex biomolecules. This compound is particularly vital for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable and high-quality building blocks for drug discovery, proteomics, and the development of antibody-drug conjugates (ADCs).

Application

• Peptide Synthesis: Serves as a key activated intermediate for the controlled incorporation of the hippuric acid (Bz-Gly) moiety or other protected amino acids during solid-phase or solution-phase peptide assembly.
• Bioconjugation & ADC Development: Used to functionalize proteins, antibodies, or other biological macromolecules by creating stable amide linkages, a fundamental step in producing targeted therapeutics like Antibody-Drug Conjugates.
• Protease Substrate & Inhibitor Design: Acts as a core component in the synthesis of fluorogenic or chromogenic substrates, as well as irreversible inhibitors, for studying enzyme kinetics and activity, particularly for proteases like carboxypeptidase A.
• Cross-linking Agent: Facilitates the covalent cross-linking of amines between different molecules or surfaces, useful in material science and for immobilizing biomolecules on solid supports.
• Organic Synthesis Intermediate: Employed in medicinal chemistry for the synthesis of novel compounds and complex molecular architectures where a protected glycine derivative is required.
• Diagnostic Reagent Manufacturing: Utilized in the production of reagents for clinical diagnostics and biochemical assays that rely on specific antibody or enzyme labeling.

Basic Information

Product Name	Hippuryl n-Hydroxysuccinimide Ester
CAS No.	66134-74-5
Molecular Formula	C15H16N2O6
Molecular Weight	320.30 g/mol
Synonyms	Hippuryl N-Hydroxysuccinimide Ester; Hippuryl NHS Ester; N-Hippuryloxysuccinimide; Bz-Gly-OSu; Benzoylglycine N-Hydroxysuccinimide Ester; N-(Benzoylglycyl)oxysuccinimide; N-Succinimidyl Hippurate; Hippuric Acid N-Hydroxysuccinimide Ester
EINECS	Contact for details

Quality Control

Our Hippuryl n-Hydroxysuccinimide Ester is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive research and production applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as assay purity, identity confirmation, and impurity profiles. We adhere to relevant industry standards and can provide material tailored to specific project requirements, including research-grade and GMP-grade materials for advanced pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMF, DMSO, acetonitrile
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.