Description

Octadecyl Rhodamine B Chloride is a cationic, lipophilic derivative of the classic xanthene dye Rhodamine B, where the carboxylic acid group is replaced by a long-chain octadecyl moiety. This structural modification is critical, as it imparts strong membrane affinity, making it an invaluable tool for labeling and tracking lipid-rich structures in biological and materials research. It is essential for researchers and industrial scientists working in advanced fluorescence-based applications. Key industries that utilize this compound include life sciences, diagnostics, and specialized materials development.

Application

• Fluorescent Cell Membrane Staining: Selectively labels plasma and organelle membranes in live and fixed cells for confocal and super-resolution microscopy.
• Lipid Droplet and Lipoprotein Imaging: Used as a vital stain to visualize neutral lipid storage and dynamics within adipocytes and other cell types.
• Liposome and Micelle Tracing: Acts as a fluorescent tag to monitor the formation, stability, and cellular uptake of lipid-based drug delivery systems.
• Counterstain in Multicolor Fluorescence: Provides a distinct red-orange emission channel, compatible with GFP and DAPI, for multiplexed imaging experiments.
• Materials Science Research: Employed in the study of polymer blends, surfactant assemblies, and other soft matter systems to probe hydrophobic domains.
• Flow Cytometry: Useful for detecting and sorting cell populations based on membrane lipid content or for tracking lipophilic particle interactions.

Basic Information

Product Name	Octadecyl Rhodamine B Chloride
CAS No.	65603-19-2
Molecular Formula	C52H73ClN2O3
Molecular Weight	809.59 g/mol
Synonyms	N-(Octadecyl)rhodamine B chloride; Rhodamine B octadecyl ester chloride; Octadecyl Rhodamine B; R18; Rhodamine B C18; Stearyl Rhodamine B Chloride; 3',6'-Bis(diethylamino)-2-(octadecylimino)spiro[isoindole-1,9'-xanthen]-3-one chloride; Lipophilic Rhodamine B dye
EINECS	Contact for details

Quality Control

Our Octadecyl Rhodamine B Chloride is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic methods for identity confirmation. A Certificate of Analysis (COA) detailing purity, absorbance/emission profiles, and other critical parameters is provided with every shipment to support your research and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This compound is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccant or under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple powder or crystals
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Max (λmax)	~560 nm (in methanol)
Emission Max	~590 nm (in methanol)
Loss on Drying	≤ 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.