Description

3-o-Methylfluorescein is a chemically modified derivative of fluorescein, a cornerstone fluorophore in life science research. This compound is valued for its role as a key intermediate in the synthesis of advanced fluorescent probes and enzyme substrates, particularly for detecting reactive oxygen species. It is essential for researchers and manufacturers in the fields of biochemical assay development, diagnostic reagent production, and advanced material science. The product is supplied with a guaranteed CAS No. 65144-30-1 to ensure precise identification and sourcing reliability.

Application

• Fluorescent Probe Synthesis: A critical precursor for creating sensitive probes used in cellular imaging and molecular biology.
• Enzyme Substrate Development: Used to develop substrates for enzymes like peroxidases, enabling high-throughput screening and diagnostic assays.
• Reactive Oxygen Species (ROS) Detection: Serves as a core structure for probes that detect hydrogen peroxide and other ROS in biological systems.
• Diagnostic Reagents: Employed in the manufacturing of components for clinical diagnostic kits and biosensors.
• Bioconjugation: Acts as a labeling agent that can be conjugated to antibodies, proteins, or nanoparticles for fluorescence-based applications.
• Research & Development: A valuable tool in academic and industrial R&D for studying oxidative stress and developing new analytical methods.

Basic Information

Product Name	3-o-Methylfluorescein
CAS No.	65144-30-1
Molecular Formula	C22H16O5
Molecular Weight	360.36 g/mol
Synonyms	3-O-Methylfluorescein; 3-Methoxyfluorescein; 6'-Hydroxy-3'-methoxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one; Fluorescein 3-methyl ether; 3-Methoxy-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
EINECS	Contact for details

Quality Control

Our 3-o-Methylfluorescein is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic methods for structural confirmation. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research or production specifications. Our quality commitment supports applications requiring high-purity biochemical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive (store away from light). Keep the container tightly sealed in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Loss on Drying	≤ 2.0 %
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.