Description

Neurodye Dibac4(5) is a high-purity, membrane-permeant fluorescent dye widely used as a potentiometric probe for monitoring membrane potential changes in biological systems. Its core value lies in its high sensitivity and rapid response, enabling real-time, non-invasive measurement of cellular electrical activity. This product is essential for researchers and manufacturers in the fields of neuroscience, cardiology, and cell physiology, as well as for diagnostic kit development.

Application

• Neuroscience Research: Real-time monitoring of neuronal action potentials and synaptic activity in live cells and tissues.
• Cardiac Electrophysiology: Assessment of cardiomyocyte membrane potential and drug-induced arrhythmogenic effects.
• High-Throughput Screening (HTS): Used in plate reader assays for drug discovery targeting ion channels and membrane receptors.
• Cellular Physiology Studies: Investigation of membrane potential dynamics in various cell types, including stem cells and insulin-secreting cells.
• Diagnostic Development: A key component in the formulation of research-use-only (RUO) and in vitro diagnostic (IVD) assay kits.
• Fluorescence Imaging: Compatible with fluorescence microscopy and flow cytometry for spatial analysis of membrane potential.

Basic Information

Product Name	Neurodye Dibac4(5)
CAS No.	63560-89-4
Molecular Formula	C26H19N2O6S- • C21H20N+
Molecular Weight	781.91 g/mol
Synonyms	bis-(1,3-Dibutylbarbituric acid)trimethine oxonol; DiBAC4(5); bis-(1,3-Dibutylbarbituric acid)pentamethine oxonol; Dibutylbarbituric Acid Trimethine Oxonol; Dibutylbarbituric Acid Pentamethine Oxonol; BBBT; Potential-Sensitive Oxonol Dye; Membrane Potential Probe
EINECS	Contact for details

Quality Control

Our Neurodye Dibac4(5) is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing to ensure high purity, batch-to-batch consistency, and performance reliability for sensitive research applications. Certificates of Analysis (COA) detailing purity, identity, and functional performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	Dark green to black crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Fluorescence Properties	Ex/Em ~490/516 nm (solvent dependent)
Functional Performance	Validated in membrane potential assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.