Description

Resazurin Sodium Salt is a vital redox indicator and biochemical reagent widely used in cell viability and bacterial metabolic activity assays. Its core value lies in its ability to undergo a distinct colorimetric change from blue to pink/colorless upon reduction, providing a clear, measurable signal. This makes it indispensable for researchers and quality control professionals in the pharmaceutical, biotechnology, and food safety industries who require reliable, high-purity reagents for microbiological testing.

Application

• Microbiological Assays: Primary application as a redox indicator in resazurin reduction tests (e.g., alamarBlue™) for measuring cell proliferation, cytotoxicity, and bacterial enumeration.
• Milk Quality Testing: Key component in the resazurin test for assessing the microbiological quality and shelf-life of raw milk.
• Antimicrobial Susceptibility Testing (AST): Used in colorimetric methods to determine the minimum inhibitory concentration (MIC) of antibiotics against bacterial and fungal pathogens.
• Biofilm Research: Employed to quantify metabolic activity within microbial biofilms in industrial and clinical settings.
• Enzyme Activity Assays: Serves as an electron acceptor in assays for oxidoreductase enzymes like diaphorase.
• Industrial Bioprocess Monitoring: Used to monitor microbial contamination and metabolic health in fermentation processes and fuel ethanol production.
• Toxicity Screening: Applied in environmental monitoring to assess the toxic effects of chemicals or pollutants on microbial and mammalian cells.

Basic Information

Product Name	Resazurin Sodium Salt
CAS No.	62758-13-8
Molecular Formula	C12H6NNaO4
Molecular Weight	251.17 g/mol
Synonyms	Resazurin, sodium salt; 7-Hydroxy-3H-phenoxazin-3-one 10-oxide sodium salt; AlamarBlue™ reagent (key component); Diazo-resorcinol sulfonate sodium salt; Azoresorcin; NSC 403697; EINECS 263-717-5

Quality Control

Our Resazurin Sodium Salt is manufactured under a strict quality management system. Each batch undergoes rigorous analytical testing, including HPLC for purity, to ensure it meets the high standards required for sensitive biological assays. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and performance data is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to maintain stability and performance.

Specification

Item	Specification
Appearance	Dark red to brownish-red powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 5.0 %
Absorbance Ratio (A600/A380)	≥ 1.5 (in phosphate buffer)
Biological Activity	Conforms to reference standard in cell viability assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.