Description

Rhodamine 123 is a cell-permeant, cationic, green-fluorescent dye widely used as a selective stain for mitochondria in live cells. Its value lies in its ability to accumulate in active mitochondria based on membrane potential, making it a critical tool for assessing cell viability and mitochondrial function. This compound is essential for researchers and industrial scientists in biotechnology, pharmaceuticals, and life sciences, particularly for applications in flow cytometry, fluorescence microscopy, and apoptosis studies.

Application

• Mitochondrial Membrane Potential Assay: A vital fluorescent probe for measuring mitochondrial health and activity in live cells.
• Flow Cytometry & Cell Sorting: Used to stain and identify populations of cells based on their metabolic state.
• Fluorescence Microscopy: Enables visualization of mitochondrial morphology and distribution within cells.
• Apoptosis (Programmed Cell Death) Research: Monitors early-stage apoptosis through changes in mitochondrial potential.
• Cancer Research: Studies drug effects on cancer cell metabolism and viability.
• Toxicology Screening: Assesses the impact of chemical compounds on cellular energy production.
• Stem Cell Research: Evaluates the metabolic status and differentiation potential of stem cells.

Basic Information

Product Name	Rhodamine 123
CAS No.	62669-70-9
Molecular Formula	C21H17ClN2O3
Molecular Weight	380.82 g/mol
Synonyms	Rhodamine 123; 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester hydrochloride; Basic Violet 11; R123; Rh 123; Mitotracker Green; Mitochondrial Green FM; 3,6-Diamino-9-[2-(methoxycarbonyl)phenyl]xanthylium chloride
EINECS	Contact for details

Quality Control

Our Rhodamine 123 is produced under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive biological assays. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence profiles, and performance data is provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Absorbance (Max)	~507 nm
Fluorescence (Max)	~529 nm
Loss on Drying	≤ 2.0 %
Solubility	Soluble in DMSO, Ethanol, Methanol
Biological Performance	Suitable for mitochondrial staining

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.