Description

Ethidium Homodimer is a high-affinity, cell-impermeant nucleic acid stain widely used as a viability indicator. Its core value lies in its ability to selectively bind to the DNA of membrane-compromised cells, producing a strong fluorescent signal that is essential for accurate dead cell quantification. This product is critical for researchers and quality control professionals in biotechnology, pharmaceutical development, and cell-based assay manufacturing who require reliable and sensitive tools for cytotoxicity testing and apoptosis studies.

Application

• Cell Viability and Cytotoxicity Assays: Primary stain for distinguishing live from dead cells in mammalian, bacterial, and yeast cultures.
• Apoptosis/Necrosis Detection: Used in combination with other fluorescent probes to differentiate between apoptotic and necrotic cell death pathways.
• Microbiological Research: Assessing bacterial cell membrane integrity and enumerating non-viable bacteria in environmental and clinical samples.
• High-Content Screening (HCS): A key reagent in automated imaging platforms for drug discovery and toxicology screening.
• Flow Cytometry: Standard fluorescent marker for dead cell exclusion in multiparametric flow cytometric analysis.
• Biofilm Studies: Evaluating the viability of cells within microbial biofilm structures.

Basic Information

Product Name	Ethidium Homodimer
CAS No.	61926-22-5
Molecular Formula	C₅₁H₆₀Br₄N₆
Molecular Weight	1129.59 g/mol
Synonyms	EthD-1; Ethidium Homodimer-1; Ethidium Homodimer I; Homodimeric Ethidium; 3,8-Diamino-5,3'-diethyl-6-phenyl-1,1'-(1,3-propanediyl)bis[phenanthridinium] Dibromide; 1,1'-(1,3-Propanediyl)bis[(3,8-diamino-6-phenyl-5-ethylphenanthridinium)] Dibromide; Bis-Ethidium; Nucleic Acid Stain
EINECS	Contact for details

Quality Control

Our Ethidium Homodimer is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science research. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and performance in standard assay formats is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Red to dark red powder
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorbance (Maxima)	~528 nm in aqueous buffer
Fluorescence (Emission Max)	~617 nm when bound to DNA
Solubility	Soluble in water, DMSO, DMF
Loss on Drying	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.