Description

Eosin 5-Isothiocyanate is a reactive fluorescent dye derivative of eosin, widely used as a labeling reagent for proteins and other biomolecules. Its core value lies in its ability to form stable covalent bonds with primary amines, enabling the preparation of highly fluorescent conjugates for sensitive detection and analysis. This compound is essential for researchers and manufacturers in the fields of life sciences, diagnostics, and biotechnology, particularly for applications in fluorescence microscopy, flow cytometry, and immunoassay development.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to primary amine groups (e.g., lysine residues) for creating fluorescent probes.
• Cell and Tissue Staining: Used as a fluorescent tag in histology and fluorescence microscopy for visualizing cellular structures.
• Flow Cytometry: Preparation of fluorescently-labeled antibodies and other ligands for cell surface marker analysis.
• Immunoassay Development: Key reagent for developing fluorescent ELISA and other immunoassay detection systems.
• Bioconjugation and Chemical Biology: Tool for studying protein-protein interactions, localization, and dynamics.
• Diagnostic Reagent Manufacturing: Component in the production of in-vitro diagnostic (IVD) kits and reagents.

Basic Information

Product Name	Eosin 5-Isothiocyanate
CAS No.	60520-47-0
Molecular Formula	C21H9NO7S2
Molecular Weight	475.43 g/mol
Synonyms	EITC; Eosin Isothiocyanate; Eosin 5-ITC; 2-(2,4,5,7-Tetrabromo-6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid isothiocyanate; 5-Isothiocyanatofluorescein; Tetrabromoeosin isothiocyanate; Eosin-5-isothiocyanate isomer; Reactive Red 4 (related dye class)
EINECS	Contact for details

Quality Control

Our Eosin 5-Isothiocyanate is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification (UV-Vis, IR), and functional testing for labeling efficiency. We provide Certificates of Analysis (COA) with each shipment, detailing all relevant specifications. Our quality commitment ensures the reproducibility and performance required for critical research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Red to reddish-brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	525 - 535 nm
Emission Maximum	545 - 555 nm
Loss on Drying	≤ 2.0 %
Functional Activity (Labeling)	Conforms to reference

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.