Description

Tetramethylrosamine Chloride is a high-purity, cationic fluorescent dye widely used as a vital stain and probe in biological research. Its primary value lies in its ability to selectively stain mitochondria in live cells, providing critical insights into cellular health, apoptosis, and metabolic activity. This product is essential for researchers and manufacturers in the life sciences, biotechnology, and pharmaceutical development sectors who require reliable, high-quality reagents for fluorescence microscopy, flow cytometry, and high-content screening applications.

Application

• Mitochondrial Staining: A vital stain for visualizing and quantifying mitochondria in live mammalian cells, yeast, and other organisms.
• Apoptosis Detection: Used in assays to monitor changes in mitochondrial membrane potential, a key early indicator of programmed cell death.
• Cell Viability & Cytotoxicity Assays: Employed to assess cell health and screen for toxic compounds based on mitochondrial function.
• Flow Cytometry: A fluorescent probe for analyzing mitochondrial mass and activity in cell populations.
• High-Content Screening (HCS): A key reagent in automated imaging platforms for drug discovery and phenotypic screening.
• Neuroscience Research: Used to study mitochondrial dynamics and function in neurons and other specialized cell types.
• Microbiology: Applied to study mitochondrial function and membrane potential in fungi and protozoa.

Basic Information

Product Name	Tetramethylrosamine Chloride
CAS No.	6837-70-3
Molecular Formula	C25H25ClN2O3
Molecular Weight	436.93 g/mol
Synonyms	Tetramethylrosamine, Methyl Ester, Chloride; TMRM; Tetramethylrhodamine Methyl Ester Perchlorate; TMRE; TMR; 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester chloride; Rhodamine 6G derivative; Mitochondrial stain TMRM; Fluorescent mitochondrial probe.
EINECS	Contact for details

Quality Control

Our Tetramethylrosamine Chloride is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance in sensitive biological applications. Each lot is characterized by HPLC for purity and undergoes functional validation in cell-based assays. A comprehensive Certificate of Analysis (COA) detailing identity, purity, absorbance/fluorescence profiles, and performance data is provided with every shipment to support your research and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. For long-term storage, desiccate at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. The material is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Dark red to purple crystalline powder
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~548 nm (in methanol)
Emission Maximum (λem)	~573 nm (in methanol)
Extinction Coefficient (ε)	~95,000 cm-1M-1
Solubility	Soluble in DMSO, DMF, methanol; sparingly soluble in water
Loss on Drying	≤ 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.