Description

Tetramethylrhodamine B Isothiocyanate is a reactive fluorescent dye derivative widely used as a labeling reagent for proteins, antibodies, and nucleic acids. Its core value lies in its high fluorescence quantum yield and excellent photostability, enabling sensitive detection and imaging in complex biological systems. This product is essential for researchers and manufacturers in the fields of bioconjugation, flow cytometry, fluorescence microscopy, and immunoassay development.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation via the isothiocyanate group for use in immunofluorescence (IF) and immunohistochemistry (IHC).
• Nucleic Acid Probes: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Flow Cytometry: Preparation of fluorescently-tagged antibodies for cell surface marker analysis and intracellular staining.
• Fluorescence Microscopy: Creating probes for high-resolution cellular and tissue imaging.
• Bioconjugation & Chemical Biology: A key reagent for tagging biomolecules to study protein interactions, localization, and dynamics.
• Diagnostic Assay Development: Used in the manufacture of lateral flow assays and other diagnostic kits requiring a bright, stable fluorescent signal.

Basic Information

Product Name	Tetramethylrhodamine B Isothiocyanate
CAS No.	6749-36-6
Molecular Formula	C25H21N3O3S
Molecular Weight	443.52 g/mol
Synonyms	5(6)-Tetramethylrhodamine B Isothiocyanate; 5(6)-TAMRA Isothiocyanate; Tetramethylrhodamine B Isothiocyanate Mixed Isomers; Rhodamine B Isothiocyanate; TRITC; 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester isomer; 4,7-Dichloro-5(6)-TAMRA; X-Rhodamine Isothiocyanate (common for mixed isomers)
EINECS	Contact for details

Quality Control

Our Tetramethylrhodamine B Isothiocyanate is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for life science research and in-vitro diagnostic applications. Certificates of Analysis (COA) detailing purity, fluorescence properties, and performance data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 90% (Mixed Isomers)
Absorption Maximum (λmax)	~550 nm
Emission Maximum	~575 nm
Extinction Coefficient (ε)	Contact for details
Fluorescence Quantum Yield (φ)	Contact for details
Water Content (KF)	< 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.