Description

Toluidine Blue For Microscopy is a metachromatic cationic dye belonging to the thiazine class, widely recognized for its specific staining properties in biological and histological applications. Its primary value lies in its ability to selectively stain acidic tissue components, such as mast cell granules and cartilage, providing critical contrast and diagnostic clarity under a microscope. This product is essential for research laboratories, clinical pathology departments, and educational institutions engaged in life sciences and medical diagnostics.

Application

• Histological Staining: Primary application for staining mast cells, cartilage, and mucopolysaccharides (e.g., in toluidine blue O staining protocols).
• Cytological Analysis: Used in cytology smears for the identification of abnormal cells, including in oral cancer screening.
• Nissl Staining: Employed in neuroscience to stain rough endoplasmic reticulum (Nissl bodies) in neuronal cell bodies.
• Microbiological Staining: Acts as a component in certain bacterial staining techniques to enhance visualization.
• Metachromatic Staining: Key dye for demonstrating metachromasia in tissues containing polyanions like glycosaminoglycans.
• Educational Microscopy: Standard dye used in teaching laboratories for introductory histology and cell biology courses.

Basic Information

Product Name	Toluidine Blue For Microscopy
CAS No.	6586-04-5
Molecular Formula	C15H16ClN3S
Molecular Weight	305.83 g/mol
Synonyms	Toluidine Blue O; Basic Blue 17; Tolonium Chloride; C.I. 52040; 3-Amino-7-(dimethylamino)-2-methylphenothiazin-5-ium chloride; Dimethyltoluthionine chloride; TBO
EINECS	229-503-3

Quality Control

Our Toluidine Blue For Microscopy is manufactured and tested to meet the stringent requirements for biological staining applications. Each batch is subjected to rigorous quality testing, including spectrophotometric analysis for dye content and purity, to ensure consistent metachromatic performance and batch-to-batch reproducibility. A Certificate of Analysis (COA) detailing identity, purity, and performance in standard staining protocols is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at a controlled room temperature (15-25°C) in a dry environment. The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	Dark green powder with bronze luster
Identification (IR)	Conforms to structure
Assay (Dye Content)	≥ 85%
Loss on Drying	≤ 5.0%
Solubility	Soluble in water, sparingly soluble in ethanol
Absorption Maximum (λmax)	630 - 635 nm (in water)
Performance Test	Meets staining criteria for mast cell granules

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.