Description

Toluidine Blue O for microscopy is a metachromatic cationic dye belonging to the thiazine class, widely recognized for its critical role in biological and histological staining. Its primary value lies in its ability to selectively stain acidic tissue components, such as cartilage, mast cell granules, and nucleic acids, providing exceptional contrast and detail under the microscope. This product is essential for professionals in life sciences, clinical diagnostics, and academic research who require reliable, high-quality stains for precise cellular and tissue analysis. Toluidine Blue O CAS NO 6568-04-5 is a fundamental tool for advancing scientific discovery and diagnostic accuracy.

Application

• Histological Staining: Used for metachromatic staining of cartilage, mast cells, and mucopolysaccharides in tissue sections.
• Cytological Analysis: Employed in staining blood smears, bone marrow aspirates, and other cytological preparations to highlight cellular structures.
• Nucleic Acid Detection: Useful for staining DNA and RNA in gel electrophoresis and in situ hybridization techniques.
• Microbiological Staining: Applied in the identification of certain bacteria and fungi based on their cell wall composition.
• Dermatological Procedures: Utilized as a vital stain in procedures like toluidine blue staining for the early detection of oral and esophageal malignancies.
• Neuroscience Research: Aids in staining neural tissue, particularly for visualizing Nissl substance in neurons.
• Quality Control in Manufacturing: Serves as a staining agent in the quality assessment of pharmaceutical and biotechnological products.

Basic Information

Product Name	Toluidine Blue O for Microscopy
CAS No.	6568-04-5
Molecular Formula	C15H16ClN3S
Molecular Weight	305.82 g/mol
Synonyms	Toluidine Blue O; Tolonium Chloride; Basic Blue 17; C.I. 52040; Toluidine Blue; Methyltoluidine Blue; TBO; 3-Amino-7-(dimethylamino)-2-methylphenothiazin-5-ium chloride
EINECS	229-503-3

Quality Control

Our Toluidine Blue O for microscopy is manufactured under strict quality management systems to ensure batch-to-batch consistency and performance. Each lot undergoes comprehensive analytical testing, including identity confirmation, purity assay, and verification of staining performance. Certificates of Analysis (COA) detailing all test results are available upon request to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability and performance.

Specification

Item	Specification
Appearance	Dark green powder with metallic luster
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 5.0 %
Solubility	Soluble in water and ethanol
Absorption Maximum	630 - 640 nm (in water)
Staining Performance	Meets defined biological staining criteria

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.