Description

Eosin Methylene-Blue CAS NO 6359-04-2 is a vital biological stain and diagnostic reagent composed of a mixture of eosin Y and methylene blue. This compound is essential for differentiating bacterial types based on their metabolic properties, providing critical visual contrast in microbiological assays. It is a key consumable for microbiology laboratories, clinical diagnostics, and pharmaceutical quality control departments worldwide.

Application

• Microbiological Culture Media: Primary component in Levine's Eosin Methylene Blue (EMB) agar for the selective and differential isolation of Gram-negative bacteria, particularly coliforms like E. coli and Klebsiella.
• Clinical Diagnostics: Used in staining procedures to identify and characterize enteric pathogens in water, food, and clinical samples.
• Hematology and Histology: Employed as a polychrome stain for blood films and tissue sections to enhance cellular detail under microscopic examination.
• Pharmaceutical Testing: Applied in sterility testing and microbial limit tests for non-sterile products as per pharmacopeial guidelines (e.g., USP, EP).
• Environmental Monitoring: A standard reagent in methods for the detection and enumeration of fecal coliforms in water supplies and environmental samples.
• Educational and Research Laboratories: Fundamental staining reagent for teaching microbiology and conducting bacteriological research.

Basic Information

Product Name	Eosin Methylene-Blue
CAS No.	6359-04-2
Molecular Formula	Mixture of C20H6Br4Na2O5 (Eosin Y) and C16H18ClN3S (Methylene Blue)
Molecular Weight	Mixture
Synonyms	EMB Stain; Eosin Methylene Blue Stain; Levine's Eosin Methylene Blue; Eosin-Methylene Blue Mixture; Eosin Methylene Blue Agar Component; Eosin Methylene Blue Dye; Eosin Methylene Blue Solution; Eosin-Methylene Blue, Certified
EINECS	Contact for details

Quality Control

Our Eosin Methylene-Blue is manufactured and tested to meet the stringent requirements for diagnostic and microbiological applications. Each batch undergoes comprehensive analysis to ensure consistent staining performance, spectral properties, and purity. Certificates of Analysis (COA) detailing identity, strength, and performance in standard microbiological assays are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Keep the container tightly sealed to protect from moisture.

Specification

Item	Specification
Appearance	Green crystalline powder with bronze luster
Identification (IR)	Conforms to reference spectrum
Absorbance Spectrum	Conforms to reference
Staining Performance (EMB Agar)	Produces characteristic metallic sheen with E. coli
Solubility (in Water)	Complete, clear solution
Loss on Drying	≤ 5.0%
Ash Content	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.