Description

Coomassie™ Brilliant Blue G-250 is a high-purity, triphenylmethane dye widely recognized as a critical reagent in protein analysis. Its primary value lies in its exceptional sensitivity and reliability for the colorimetric detection and quantification of proteins, most notably in the Bradford assay. This product is essential for research laboratories, diagnostic kit manufacturers, and biopharmaceutical quality control units requiring precise and consistent protein measurement. Its consistent performance makes it a staple in life science research and industrial biotechnology processes.

Application

• Protein Quantification (Bradford Assay): The predominant application as a rapid, sensitive dye-binding reagent for determining protein concentration in solution.
• Life Science Research: Used in biochemistry, molecular biology, and cell culture labs for standardizing experiments and analyzing protein samples.
• Diagnostic Manufacturing: A key component in the production of clinical diagnostic kits and reagents for medical testing.
• Biopharmaceutical QC: Employed in quality control testing during the development and production of therapeutic proteins and vaccines.
• Gel Staining (Coomassie Staining): Used in analytical techniques like SDS-PAGE to visualize protein bands after electrophoresis.
• Food & Beverage Analysis: Applied in the analysis of protein content in food products for nutritional labeling and quality assurance.

Basic Information

Product Name	Coomassie™ Brilliant Blue G-250
CAS No.	6104-58-2
Molecular Formula	C47H48N3O7S2Na
Molecular Weight	854.02 g/mol
Synonyms	Brilliant Blue G; Acid Blue 90; Coomassie Brilliant Blue G; C.I. 42655; Serva Blue G; Xylene Brilliant Cyanine G; Aniline Blue, water soluble; Brilliant Blue G 250; Coomassie® Brilliant Blue G-250
EINECS	228-058-2

Quality Control

Our Coomassie Brilliant Blue G-250 is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is tested to meet stringent specifications for identity, purity, and dye content. Certificates of Analysis (COA) detailing specific test results are provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed when not in use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	Dark green to blue-green powder
Identification (IR)	Conforms to structure
Assay (Dye Content)	≥ 90%
Absorbance Ratio (A610/A470)	≥ 1.5
Loss on Drying	≤ 5.0 %
Solubility (in Water)	Clear, intense blue solution
pH (1% Solution)	Contact for details
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.