Description

Resorufin is a high-purity, synthetic heterocyclic organic compound widely utilized as a critical fluorescent probe and biochemical reagent. Its unique photophysical properties make it an essential tool for enzymatic assays, cellular imaging, and diagnostic research, providing high sensitivity and specificity. This product is primarily required by research institutions, pharmaceutical developers, and diagnostic kit manufacturers engaged in advanced life science and analytical chemistry applications.

Application

• Enzymatic Activity Assays: Used as a fluorogenic substrate for detecting the activity of enzymes like cytochrome P450, esterases, and peroxidases.
• Cell Viability & Cytotoxicity Testing: Employed in live-cell imaging and assays (e.g., resazurin reduction assays) to measure metabolic activity.
• Oxidative Stress & Redox Biology Research: Acts as an indicator for reactive oxygen species (ROS) and cellular redox state monitoring.
• Diagnostic Reagent Manufacturing: Incorporated as a key component in the development of clinical diagnostic kits and biosensors.
• Fluorescence Microscopy & Imaging: Serves as a fluorescent tracer or tag in biological staining and microscopy applications.
• Analytical Chemistry Standard: Used as a reference standard in HPLC and other chromatographic methods for quality control.
• Biochemical Pathway Studies: Applied in research focused on metabolic pathways, drug metabolism, and enzyme kinetics.
• High-Throughput Screening (HTS): Utilized in pharmaceutical discovery for screening compound libraries against enzymatic targets.

Basic Information

Product Name	Resorufin
CAS No.	635-78-9
Molecular Formula	C₁₂H₇NO₃
Molecular Weight	213.19 g/mol
Synonyms	7-Hydroxy-3H-phenoxazin-3-one; 7-Hydroxyphenoxazin-3-one; Resorufine; Phenoxazin-3-one, 7-hydroxy-; NSC 403169; AI3-52236; C.I. 57230
EINECS	211-247-1

Quality Control

Our Resorufin is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by FT-IR and NMR spectroscopy. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Orange to dark red powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0 %
Residue on Ignition	≤ 0.1 %
Fluorescence Properties	Conforms to standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.