Description

Acridine Orange CAS NO 65-61-2 is a versatile metachromatic fluorescent dye and a vital nucleic acid-selective cationic probe. Its core value lies in its ability to differentially stain DNA and RNA, emitting distinct fluorescence colors that are crucial for cellular analysis. This compound is essential for researchers and quality control laboratories in the biotechnology, pharmaceutical, and diagnostic sectors requiring precise nucleic acid visualization and quantification.

Application

• Fluorescence Microscopy & Cell Staining: Used as a vital stain for visualizing DNA and RNA in live and fixed cells, bacteria, and parasites.
• Flow Cytometry: Employed in cell cycle analysis and apoptosis detection due to its differential binding to nucleic acids.
• Microbiological Research: Applied for the detection and enumeration of microorganisms in environmental and clinical samples.
• Histopathology: Serves as a fluorescent counterstain in tissue sections to highlight cellular structures.
• Biochemical Assays: Utilized in quantitative assays for DNA and RNA based on fluorescence enhancement.
• Photodynamic Therapy (PDT) Research: Investigated for its photosensitizing properties in experimental cancer treatment studies.

Basic Information

Product Name	Acridine Orange
CAS No.	65-61-2
Molecular Formula	C17H19N3
Molecular Weight	265.36 g/mol
Synonyms	Acridine Orange Base; 3,6-Bis(dimethylamino)acridine; Basic Orange 14; C.I. 46005; Acridine Orange Free Base; Acridine Orange Stain; 3,6-Bis(dimethylamino)acridine hydrochloride (parent compound); AO; AOHCl
EINECS	200-614-0

Quality Control

Our Acridine Orange is produced under a rigorous quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopy for identity confirmation, and tests for residual solvents and heavy metals. A Certificate of Analysis (COA) detailing all specifications and results is provided to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive; prolonged exposure to light should be avoided to prevent degradation.

Specification

Item	Specification
Appearance	Orange to brown crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.5 %
Heavy Metals (as Pb)	≤ 20 ppm
Fluorescence	Conforms to reference

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.