Description

5-Carboxyfluorescein-Azide is a high-purity, bifunctional fluorescent labeling reagent that combines a fluorescein-based fluorophore with a reactive azide group. This compound is critical for bioorthogonal click chemistry applications, enabling highly specific and efficient conjugation to alkyne-modified biomolecules under physiological conditions. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, proteomics, and advanced diagnostics for labeling proteins, antibodies, nucleic acids, and cellular components.

Application

• Bioconjugation & Protein Labeling: Site-specific fluorescent labeling of antibodies, enzymes, and other proteins via copper-catalyzed or strain-promoted azide-alkyne cycloaddition (CuAAC/SPAAC).
• Cell Imaging & Tracking: Visualization of cellular structures, receptor trafficking, and intracellular processes when conjugated to alkyne-tagged probes or metabolites.
• Diagnostic Assay Development: Synthesis of fluorescent detection reagents for ELISA, lateral flow assays, and in situ hybridization to enhance sensitivity and specificity.
• Materials Science & Surface Modification: Functionalization of nanoparticles, polymers, and biosensor surfaces with fluorescent tags for analytical and biomedical devices.
• Nucleic Acid Labeling: Preparation of fluorescently tagged oligonucleotides and probes for genomics and molecular biology applications.
• Drug Discovery & High-Throughput Screening (HTS): Development of fluorescent tracers and labeled ligands for target engagement and binding studies.

Basic Information

Product Name	5-Carboxyfluorescein-Azide
CAS No.	510758-23-3
Molecular Formula	C25H15N3O7
Molecular Weight	469.41 g/mol
Synonyms	5-FAM Azide; 5-Carboxyfluorescein N3; 6-Carboxyfluorescein Azide (regioisomer); 5(6)-Carboxyfluorescein Azide; 5-FAM-N3; Fluorescein-5-Azide; 5-CF Azide; Azido-Fluorescein
EINECS	Contact for details

Quality Control

Our 5-Carboxyfluorescein-Azide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional validation in conjugation assays. A detailed Certificate of Analysis (COA) documenting identity, purity, and performance is provided with every shipment to ensure compliance with research and diagnostic-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Functional Performance	Conforms (by conjugation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.