Description

2,5-Dioxopyrrolidin-1-yl Docosanoate is a high-purity, long-chain fatty acid-derived active ester, specifically the N-hydroxysuccinimide (NHS) ester of behenic acid. This compound serves as a critical reagent for the efficient, zero-length crosslinking and labeling of primary amines in peptides, proteins, and other biomolecules under mild aqueous conditions. It is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and advanced materials sectors who require reliable, high-yield bioconjugation and surface modification strategies.

Application

• Bioconjugation Reagent: Primary application for covalently attaching labels (e.g., fluorophores, biotin), drugs, or other molecules to proteins, antibodies, and peptides via amine groups.
• Surface Functionalization: Used to modify the surface of nanoparticles, microspheres, biosensors, and microarray slides with long-chain hydrophobic or functional groups for specific binding assays.
• Polymer & Material Science: Acts as a reactive intermediate in the synthesis of functional polymers, dendrimers, and smart materials requiring amine-reactive sites.
• Proteomics & Biomarker Research: Employed in sample preparation for mass spectrometry, including isotopic labeling for quantitative proteomic analysis (e.g., ICAT, iTRAQ-like strategies).
• Diagnostic Kit Manufacturing: A key component in the production of enzyme-linked immunosorbent assay (ELISA) kits and other immunoassays for immobilizing capture antibodies or antigens.
• Lipid & Membrane Research: Useful for synthesizing labeled lipid derivatives to study membrane dynamics, lipid-protein interactions, and cellular uptake mechanisms.

Basic Information

Product Name	2,5-Dioxopyrrolidin-1-yl Docosanoate
CAS No.	58047-86-2
Molecular Formula	C₂₆H₄₇NO₄
Molecular Weight	437.66 g/mol
Synonyms	NHS-Behenate; Behenic Acid N-Hydroxysuccinimide Ester; Docosanoic Acid 2,5-Dioxo-1-pyrrolidinyl Ester; N-Succinimidyl Behenate; 1-[(Docosanoyloxy)-2,5-pyrrolidinedione]; N-Hydroxysuccinimide Behenic Acid Ester; Behenic Acid NHS Ester; C22:0 NHS Ester
EINECS	Contact for details

Quality Control

Our 2,5-Dioxopyrrolidin-1-yl Docosanoate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and loss on drying for moisture content, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider storing under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white crystalline powder or solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0% (Area %)
Melting Point	Contact for details
Water Content (KF)	≤ 1.0 %
Solubility	Soluble in DMF, DMSO, acetonitrile; sparingly soluble in alcohols; insoluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.