Description

Boc-Cit-Onp is a protected amino acid derivative, specifically the p-nitrophenyl ester of N-Boc-L-citrulline. This compound is a crucial building block in solid-phase peptide synthesis (SPPS), where it facilitates the efficient and controlled incorporation of the modified amino acid citrulline into peptide chains. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of peptides for therapeutic, diagnostic, and biochemical research applications, particularly in studies involving post-translational modifications like citrullination.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key reagent for the controlled, sequential addition of Boc-protected citrulline during automated or manual peptide assembly.
• Peptide Drug Discovery: Used in the research and development of novel peptide-based therapeutics targeting various diseases.
• Biochemical Research: Essential for synthesizing citrullinated peptides to study autoimmune diseases like rheumatoid arthritis, where citrullination is a critical antigenic process.
• Antibody and Diagnostic Reagent Production: Employed to create specific antigens for generating antibodies against citrullinated proteins.
• Enzyme Substrate Synthesis: Used to prepare substrates for studying enzymes like peptidylarginine deiminases (PADs).
• Custom Peptide Service Providers: A fundamental raw material for companies offering bespoke peptide synthesis services to the research community.

Basic Information

Product Name	Boc-Cit-Onp
CAS No.	56612-88-5
Molecular Formula	C₁₇H₂₃N₃O₇
Molecular Weight	381.38 g/mol
Synonyms	N-α-Boc-N-δ-Cbz-L-citrulline p-nitrophenyl ester; Boc-Cit(Z)-ONp; tert-Butyloxycarbonyl-N-δ-carbobenzoxy-L-citrulline 4-nitrophenyl ester; Boc-L-Citrulline(Cbz)-ONp; N-Boc-N'-Cbz-L-citrulline p-nitrophenyl ester; (S)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-5-((tert-butoxycarbonyl)amino)pentanoic acid; Fmoc-Cit(Boc)-OH (Note: This is the Fmoc-protected analog); A protected citrulline derivative
EINECS	Contact for details

Quality Control

Our Boc-Cit-Onp is manufactured under strict quality control protocols to ensure high chemical purity and consistency, suitable for demanding synthetic applications. Each batch is analyzed using advanced techniques including HPLC for purity assessment and NMR for structural confirmation. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing specifications for assay, chiral purity, and related substances. Our quality system is designed to support GMP-oriented research and development.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥98.0%
Chiral Purity (HPLC)	≥99.0% (L-isomer)
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.