Description

1'-Trimethylsilyl-3'-o-Trimethylsilyl-2'-Deoxy-5'-Uridylic Acid Bis(Trimethylsilyl) Ester is a highly specialized, protected nucleoside derivative crucial for advanced oligonucleotide synthesis and medicinal chemistry research. This compound serves as a key synthetic intermediate, enabling the precise construction and modification of nucleic acid sequences by protecting reactive hydroxyl groups. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNA, and other novel therapeutic modalities. The product's design ensures compatibility with automated solid-phase synthesis, offering researchers a reliable building block for cutting-edge genetic and antiviral research programs.

Application

• Oligonucleotide Synthesis: A critical phosphoramidite precursor for the solid-phase synthesis of modified DNA and RNA strands in research and therapeutic development.
• Antisense Technology: Used in the manufacture of antisense oligonucleotides designed to target specific mRNA sequences for therapeutic intervention.
• siRNA and miRNA Research: Serves as a building block for creating small interfering RNA (siRNA) and microRNA (miRNA) analogs with enhanced stability and delivery properties.
• Medicinal Chemistry: Employed as a key intermediate in the discovery and development of nucleoside-based antiviral and anticancer prodrugs.
• Diagnostic Probe Development: Utilized for synthesizing labeled nucleic acid probes used in diagnostic assays and genomic research.
• Chemical Biology Studies: Facilitates the study of nucleic acid structure, function, and protein interactions through site-specific incorporation.

Basic Information

Product Name	1'-Trimethylsilyl-3'-o-Trimethylsilyl-2'-Deoxy-5'-Uridylic Acid Bis(Trimethylsilyl) Ester
CAS No.	56211-37-1
Molecular Formula	C22H46N2O7Si4
Molecular Weight	586.98 g/mol
Synonyms	5'-O-(Bis(trimethylsilyl))-2'-deoxy-3'-O-(trimethylsilyl)uridine 1'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 2'-Deoxy-5'-uridylic Acid, 1'-(trimethylsilyl)-3'-o-(trimethylsilyl)-, Bis(trimethylsilyl) Ester; Protected 2'-Deoxyuridine Phosphoramidite; dU Silyl Protected Intermediate; Uridine, 2'-deoxy-3'-O-(trimethylsilyl)-5'-O-[bis(trimethylsilyl) phosphoryl]-, 1'-(trimethylsilyl) derivative
EINECS	Contact for details

Quality Control

Our production of this high-purity synthetic intermediate adheres to stringent GMP-like guidelines to ensure batch-to-batch consistency for sensitive research applications. Every lot is characterized using advanced analytical techniques including HPLC, NMR (¹H and ³¹P), and mass spectrometry to confirm identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment, supporting your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed under an inert atmosphere (argon or nitrogen) when not in use to prevent decomposition. For long-term storage, consider storing at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.