Description

Calcein Blue is a high-purity, fluorescent metal ion indicator and chelator derived from the calcein family. This compound is valued for its strong, stable fluorescence and high selectivity, particularly for zinc and aluminum ions, making it a critical tool in analytical and life science research. It is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and environmental monitoring sectors requiring precise metal ion detection and quantification.

Application

• Fluorescent Microscopy & Cell Biology: Used as a vital stain for visualizing intracellular zinc ion (Zn²⁺) dynamics and localization in live and fixed cells.
• Analytical Chemistry & Assay Development: Serves as a sensitive fluorescent probe for the detection and quantification of aluminum (Al³⁺) and zinc ions in various sample matrices.
• Metal Ion Chelation Studies: Employed in research to study metal binding affinity, kinetics, and the role of metal ions in biochemical pathways.
• Environmental Testing: Applied in methods for detecting trace levels of specific metal contaminants in water and soil samples.
• Pharmaceutical Research: Utilized in high-throughput screening (HTS) assays and in studying metalloprotein interactions and enzyme kinetics.
• Material Science: Can be incorporated into sensor materials and films for the development of optical metal ion sensors.

Basic Information

Product Name	Calcein Blue
CAS No.	54375-47-2
Molecular Formula	C₃₀H₂₆N₂O₁₃
Molecular Weight	622.53 g/mol
Synonyms	3,3'-Bis[N,N-bis(carboxymethyl)aminomethyl]-o-cresolsulfonphthalein; NSC 113992; o-Cresolphthalein Complexone; CPC; Fluorexon; Fluorescent Complexone; Metal Indicator Calcein Blue; [3,3'-Bis[N,N-bis(carboxymethyl)aminomethyl]-o-cresolsulfonphthalein]
EINECS	Contact for details

Quality Control

Our Calcein Blue is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification, and heavy metal analysis. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Orange to brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Heavy Metals	≤ 20 ppm
Fluorescence	Conforms to reference

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.