Description

n-Hydroxysuccinimidyl-4-Azidobenzoate is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable aryl azide group. This dual functionality is critical for creating stable covalent bonds between biomolecules under mild, controllable conditions. It is an essential tool for researchers and manufacturers in bioconjugation, proteomics, and materials surface modification, enabling the study of protein interactions and the development of advanced diagnostic and therapeutic platforms.

Application

• Bioconjugation and Protein Labeling: Covalently link proteins, peptides, or antibodies to other molecules (e.g., fluorophores, enzymes, solid supports) via primary amines under gentle conditions.
• Photoaffinity Labeling: Study protein-ligand interactions and receptor mapping by using UV light to activate the aryl azide, forming covalent bonds with nearby molecules.
• Surface Immobilization: Functionalize biosensor chips, microarray slides, or nanoparticles with biomolecules for diagnostic assays and drug delivery systems.
• Polymer and Biomaterial Modification: Introduce bioactive ligands or crosslink functional groups into hydrogels and polymeric materials for tissue engineering.
• Crosslinking Mass Spectrometry (XL-MS): Investigate protein structures and complex architectures by creating covalent crosslinks between interacting proteins for MS analysis.
• Antibody-Drug Conjugate (ADC) Research: Serve as a linker precursor in the development of novel therapeutic conjugates.

Basic Information

Product Name	n-Hydroxysuccinimidyl-4-Azidobenzoate
CAS No.	53053-08-0
Molecular Formula	C11H8N4O4
Molecular Weight	260.21 g/mol
Synonyms	4-Azidobenzoic Acid N-Hydroxysuccinimide Ester; NHS-4-Azidobenzoate; 4-Azidobenzoyloxysuccinimide; Succinimidyl 4-Azidobenzoate; HSAB; N-Succinimidyl 4-Azidobenzoate; SANPAH (a related, sulfonated analog); 4-Azidobenzoyl N-oxysuccinimide
EINECS	Contact for details

Quality Control

Our n-Hydroxysuccinimidyl-4-Azidobenzoate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and FT-IR for functional group verification. Certificates of Analysis (COA) with detailed specifications are provided to guarantee compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.