Description

Fluorescein Diacetate is a vital non-fluorescent precursor compound that is enzymatically hydrolyzed to produce the highly fluorescent molecule, fluorescein. This property makes it an essential tool for assessing cell viability and enzymatic activity, particularly in biological and biochemical research. It is a critical reagent for researchers and quality control professionals in the pharmaceutical, biotechnology, and diagnostic industries, where precise cellular assays are paramount.

Application

• Cell Viability and Cytotoxicity Assays: Used as a substrate for intracellular esterases in live cells; living cells convert the non-fluorescent diacetate into fluorescent fluorescein, allowing for quantification of viable cell populations.
• Enzyme Activity Studies: Serves as a standard substrate for measuring esterase and lipase activity in various biochemical and pharmacological research applications.
• Flow Cytometry and Fluorescence Microscopy: Employed as a fluorescent probe for labeling and tracking live cells in complex biological samples.
• Pharmaceutical Drug Screening: Utilized in high-throughput screening (HTS) platforms to evaluate the effects of drug candidates on cell health and metabolic function.
• Microbiological Research: Applied in assays to determine the viability of yeast, bacteria, and other microorganisms.
• Diagnostic Kit Manufacturing: Incorporated as a key component in the production of diagnostic kits for clinical and research laboratories.

Basic Information

Product Name	Fluorescein Diacetate
CAS No.	596-09-8
Molecular Formula	C24H16O7
Molecular Weight	416.38 g/mol
Synonyms	Fluorescein Diacetate; 3',6'-Diacetylfluorescein; FDA; Diacetylfluorescein; Fluorescein O,O'-diacetate; 2-(6-Acetyloxy-3-oxo-3H-xanthen-9-yl)benzoic acid acetate; NSC 403319
EINECS	209-882-5

Quality Control

Our Fluorescein Diacetate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR spectroscopy, and residual solvent analysis to ensure it meets the stringent requirements for research and diagnostic applications. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Fluorescence Suitability	Passes functional cell assay test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.