Description

Trypan Red is a vital biological stain and diagnostic agent widely used in life science research and medical diagnostics. Its primary value lies in its selective staining properties, which are essential for distinguishing viable from non-viable cells in vital staining procedures. This compound is a critical reagent for researchers and quality control laboratories in the biotechnology, pharmaceutical, and academic sectors.

Application

• Vital Staining in Cell Biology: Used to selectively stain dead or non-viable cells in suspension cultures, enabling accurate cell viability counts.
• Microbiological Research: Employed as a dye in various assays to study cell membrane integrity and permeability.
• Parasitology and Protozoology: Historically and currently used in laboratory studies for staining trypanosomes and other blood parasites.
• Tissue Culture Quality Control: An essential tool for monitoring contamination and assessing cell health in mammalian cell culture workflows.
• Dye Exclusion Tests: A key component in dye exclusion assays, a standard method for determining cell viability and cytotoxicity.
• Educational and Diagnostic Kits: Incorporated into prepared staining solutions and kits for teaching and diagnostic purposes.

Basic Information

Product Name	Trypan Red
CAS No.	574-64-1
Molecular Formula	C₃₂H₂₄N₆O₁₄S₄
Molecular Weight	960.86 g/mol
Synonyms	Trypan Red; Direct Red 14; Congo Corinth; C.I. 22155; Benzopurpurine 4B; C.I. Direct Red 14; Niagara Red 4B; Direct Red 4B; 3,3'-((3,3'-Dimethyl-(1,1'-biphenyl)-4,4'-diyl)bis(azo))bis(4-amino-1-naphthalenesulfonic acid) disodium salt
EINECS	209-358-4

Quality Control

Our Trypan Red is produced and tested under a rigorous quality management system to ensure batch-to-batch consistency and performance in sensitive biological applications. Each lot is analyzed for critical parameters including purity, dye content, and suitability for cell staining. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Keep the container tightly sealed to protect from moisture.

Specification

Item	Specification
Appearance	Dark red to brownish-red powder
Identification (IR)	Conforms to structure
Assay (Dye Content)	≥ 85%
Loss on Drying	≤ 5.0 %
Solubility (in Water)	Passes test
pH (1% Solution)	6.0 - 8.0
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.