Description

Azure B CAS NO 531-55-5 is a high-purity synthetic dye belonging to the thiazine class, widely recognized for its vibrant blue color and specific staining properties. This compound is critical for applications requiring precise colorimetric analysis and histological differentiation. It is an essential reagent for research and diagnostic laboratories, as well as for specialized industrial coloring processes. Professionals in the life sciences and chemical manufacturing sectors rely on its consistent quality and performance.

Application

• Histological Staining: Used as a vital component in Romanowsky-type stains (e.g., Giemsa, Wright's stain) for differentiating blood cells and parasites in microscopy.
• Microbiological Analysis: Employed in staining procedures for bacteria and other microorganisms to enhance visibility under a microscope.
• Cytological Studies: Applied in chromosome banding and nuclear staining techniques for genetic and pathological research.
• Textile Dyeing: Serves as a specialized dye for coloring natural and synthetic fibers in niche textile applications.
• Ink Manufacturing: Utilized in the formulation of specialized inks for security printing or artistic purposes.
• Research & Development: Acts as a standard or reagent in chemical and biochemical research projects involving dye interactions and photochemical properties.
• Diagnostic Kits: Incorporated as a key ingredient in prepared staining kits for clinical and veterinary diagnostics.
• Quality Control: Used as a reference standard in the quality control of other staining products and dyes.

Basic Information

Product Name	Azure B
CAS No.	531-55-5
Molecular Formula	C15H16ClN3S
Molecular Weight	305.83 g/mol
Synonyms	Azure B chloride; C.I. 52010; 3-Amino-7-(dimethylamino)-2-methylphenothiazin-5-ium chloride; Methylene Azure; Dimethylthionine chloride; Basic Blue 12; Toluidine Blue O (related compound); Phenothiazin-5-ium, 3-amino-7-(dimethylamino)-2-methyl-, chloride
EINECS	208-518-8

Quality Control

Every batch of Azure B is manufactured under strict quality management protocols. We provide comprehensive analytical documentation to ensure the product meets the high standards required for diagnostic and research applications. A detailed Certificate of Analysis (COA) is supplied with each shipment, reporting key parameters such as identity, purity, and absorbance characteristics. Our quality system is designed to support compliance with relevant industry practices for laboratory reagents.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Keep the container tightly sealed to protect this light-sensitive and hygroscopic material from moisture and atmospheric contamination.

Specification

Item	Specification
Appearance	Dark green to blue powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Absorbance (λ max)	648 - 652 nm
Loss on Drying	≤ 5.0 %
Residue on Ignition	≤ 1.0 %
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.