Description

Dimidium Bromide is a vital cationic phenanthridine dye and nucleic acid stain widely recognized for its role in biological research and diagnostics. Its primary commercial value lies in its high-affinity binding to nucleic acids, enabling critical applications in molecular biology and histopathology. This compound is essential for research institutions, diagnostic laboratories, and manufacturers of biological stains and kits, particularly for fluorescence-based assays and chromosome banding techniques.

Application

• Fluorescent Staining Agent: Used as a fluorescent probe for the detection and quantification of nucleic acids (DNA and RNA) in solution and in gel electrophoresis.
• Cytogenetic Analysis: Employed in chromosome banding techniques, often in combination with other dyes, to produce specific banding patterns for karyotyping and genetic disorder diagnosis.
• Histological Staining: Applied in histopathology to stain tissue sections, aiding in the visualization of cellular structures under a microscope.
• Viability Assays: Utilized in cell biology to distinguish between live and dead cells based on membrane integrity.
• Research Reagent: A key component in biochemical research kits and as a standard reagent for studying nucleic acid interactions and properties.
• Microbiological Studies: Used to stain microorganisms for enhanced visualization and counting.

Basic Information

Product Name	Dimidium Bromide
CAS No.	518-67-2
Molecular Formula	C20H18BrN3
Molecular Weight	380.28 g/mol
Synonyms	3,8-Diamino-5-methyl-6-phenylphenanthridinium bromide; Phenanthridinium, 3,8-diamino-5-methyl-6-phenyl-, bromide; Dimidium Bromide; Homidium Bromide (common name); Ethidium Bromide homologue; 3,8-Diamino-6-phenyl-5-methylphenanthridinium bromide; 6-Phenyl-5-methyl-3,8-diaminophenanthridinium bromide
EINECS	208-263-5

Quality Control

Our Dimidium Bromide is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including identity confirmation, purity assay, and impurity profiling. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting compliance with our stringent in-house specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Dark red to brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.5 %
Heavy Metals (as Pb)	≤ 20 ppm
Absorbance Ratio (Specific)	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.