Description

2-(4,5,6-Trihydroxy-3-Oxo-3H-Xanthen-9-Yl)-Benzoic Acid is a high-purity xanthene derivative, also known as Fluorescein, valued for its intense fluorescence properties. This compound serves as a critical intermediate and analytical reagent in advanced manufacturing and research sectors. It is essential for industries requiring precise colorants, sensitive detection agents, and specialized organic synthesis.

Application

• Fluorescent Tracer and Dye Manufacturing: Primary use as a precursor for synthesizing water-soluble fluorescent dyes and biological stains.
• Diagnostic Reagents: Key component in ophthalmic diagnostics (e.g., fluorescein sodium for angiography) and in vitro diagnostic kits.
• Analytical Chemistry: Employed as a pH indicator and a fluorescent probe in biochemical assays and environmental monitoring.
• Industrial Colorants: Used in the production of high-visibility inks, safety markers, and specialty pigments.
• Life Science Research: Acts as a tagging molecule in fluorescence microscopy, flow cytometry, and cellular imaging studies.
• Cosmetic and Personal Care: Utilized in the formulation of certain color cosmetics where a bright, fluorescent effect is desired.

Basic Information

Product Name	2-(4,5,6-Trihydroxy-3-Oxo-3H-Xanthen-9-Yl)-Benzoic Acid
CAS No.	518-41-2
Molecular Formula	C20H12O6
Molecular Weight	348.31 g/mol
Synonyms	Fluorescein (Free Acid); D&C Yellow No. 7; CI 45350; Solvent Yellow 94; 9-(o-Carboxyphenyl)-6-hydroxy-3H-xanthen-3-one; 3',6'-Dihydroxyfluoran; 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic Acid
EINECS	208-253-0

Quality Control

Our 2-(4,5,6-Trihydroxy-3-Oxo-3H-Xanthen-9-Yl)-Benzoic Acid is manufactured under strict quality management systems. Each batch is tested to ensure it meets stringent specifications for identity, purity, and performance. Certificates of Analysis (COA) detailing results for assay, related substances, and heavy metals are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at controlled room temperature (15-25°C). Keep away from incompatible materials. Due to its light-sensitive nature, minimize exposure during handling.

Specification

Item	Specification
Appearance	Orange-red to red-brown crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.2%
Heavy Metals (as Pb)	≤ 20 ppm
Related Substances (HPLC)	Total impurities ≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.