Description

Formazan CAS NO 504-65-4 is a vital chromogenic compound widely used as a key intermediate in biochemical assays and research. Its primary value lies in its ability to produce a measurable color change, enabling the quantitative detection of cellular metabolic activity and enzymatic processes. This product is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic kit industries, particularly for applications like cell viability testing (MTT assays) and dehydrogenase activity studies.

Application

• Cell Viability and Proliferation Assays: Critical component in the MTT assay and related tetrazolium reduction tests for measuring mitochondrial activity in live cells.
• Enzyme Activity Detection: Used as a substrate or indicator for dehydrogenase and reductase enzymes in biochemical research and diagnostic testing.
• Diagnostic Reagent Manufacturing: Serves as a key raw material in the production of clinical diagnostic kits and reagents for research laboratories.
• Microbiology and Cytotoxicity Studies: Employed in screening for antimicrobial agents and assessing the cytotoxic effects of compounds on various cell lines.
• Biochemical Research: Utilized as a colorimetric probe in a wide range of experimental protocols to monitor redox reactions and metabolic pathways.
• High-Throughput Screening (HTS): Integral to automated assay systems in drug discovery for the rapid evaluation of compound libraries.

Basic Information

Product Name	Formazan
CAS No.	504-65-4
Molecular Formula	C6H5N4O
Molecular Weight	149.13 g/mol
Synonyms	1,2,5-Triphenylformazan; Formazan, 1,2,5-triphenyl-; TPF; Triphenylformazan; 1,2,5-Triphenyl-1H-tetrazol-3-ium-5-olate; 1,3,5-Triphenylformazan; NSC 6650; CCRIS 5981
EINECS	207-995-2

Quality Control

Our Formazan is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Quality is verified through comprehensive analytical testing, including HPLC for purity, spectroscopic identification (IR, UV-Vis), and residual solvent analysis. A Certificate of Analysis (COA) detailing all specifications and test results is provided with each shipment to guarantee compliance with your research and manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Dark red to brown powder or crystals
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Melting Point	178°C - 182°C
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.