Description

Atto 655 is a near-infrared fluorescent dye belonging to the cyanine dye family, known for its high photostability and low molecular weight. This compound is essential for advanced bioanalytical applications due to its excellent fluorescence properties in the far-red to NIR spectrum, minimizing background autofluorescence and enabling deeper tissue penetration. It is a critical reagent for researchers and manufacturers in life sciences, diagnostics, and pharmaceutical development who require reliable, high-performance labeling for biomolecules.

Application

• Biomolecule Labeling: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides for fluorescence detection.
• Single-Molecule Detection (SMD): Used in super-resolution microscopy (e.g., STORM, PALM) and fluorescence correlation spectroscopy (FCS) due to its photophysical characteristics.
• Nucleic Acid Analysis: Labeling of DNA and RNA probes for in situ hybridization (FISH), PCR, and next-generation sequencing applications.
• Cell and Tissue Imaging: Fluorescent tagging for live-cell imaging, flow cytometry, and in vivo imaging in the NIR window.
• Diagnostic Assay Development: Integration into lateral flow assays, microarrays, and other diagnostic platforms as a detection label.
• Material Science: Incorporation into nanoparticles and polymers to create advanced fluorescent materials and sensors.

Basic Information

Product Name	Atto 655
CAS No.	485815-43-8
Molecular Formula	C37H47N4O7S2+
Molecular Weight	723.92 g/mol
Synonyms	ATTO 655; ATTO-655; 2-[7-[1,3-Dihydro-1,1-dimethyl-3-(4-sulfobutyl)-2H-benz[e]indol-2-ylidene]-1,3,5-heptatrienyl]-1,1-dimethyl-3-(4-sulfobutyl)-1H-benz[e]indolium Hydroxide, Inner Salt; ATTO 655 carboxylic acid; Cyanine dye ATTO 655; 1H-Benz[e]indolium, 2-[7-(1,1-dimethyl-3-(4-sulfobutyl)-1,3-dihydro-2H-benz[e]indol-2-ylidene)-1,3,5-heptatrien-1-yl]-1,1-dimethyl-3-(4-sulfobutyl)-, inner salt
EINECS	Contact for details

Quality Control

Our Atto 655 is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic methods for identity and performance verification. A detailed Certificate of Analysis (COA) documenting purity, absorbance/fluorescence properties, and functional group integrity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	663 ± 5 nm
Emission Maximum	684 ± 5 nm
Extinction Coefficient (ε)	≥ 125,000 L·mol-1·cm-1
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.