Description

Bodipy 581/591 Carboxylic Acid is a versatile, functionalized boron-dipyrromethene (BODIPY) dye derivative, widely recognized for its exceptional photostability and bright fluorescence. This compound is specifically engineered for covalent conjugation to biomolecules, enabling the creation of highly stable fluorescent probes and labels. It is an essential tool for researchers and manufacturers in the fields of bioconjugation, cellular imaging, flow cytometry, and diagnostic assay development, where reliable and intense signal generation is critical.

Application

• Fluorescent Labeling of Biomolecules: Covalent attachment to antibodies, proteins, peptides, and oligonucleotides for use in immunoassays (ELISA, Western Blot) and nucleic acid detection.
• Live-Cell and Fixed-Cell Imaging: Synthesis of organelle-specific probes or tracking dyes for confocal and super-resolution microscopy applications.
• Flow Cytometry & FACS: Development of bright, photostable fluorescent conjugates for cell surface marker detection and intracellular staining.
• FRET-Based Assays: Serving as a donor or acceptor dye in Förster Resonance Energy Transfer (FRET) pairs for studying molecular interactions.
• Polymer and Material Science: Incorporation into polymers or nanoparticles to create fluorescent materials for sensing and tracking.
• Diagnostic Kit Manufacturing: Production of key components for clinical diagnostic tests requiring high-sensitivity fluorescence detection.

Basic Information

Product Name	Bodipy 581/591 Carboxylic Acid
CAS No.	480999-04-0
Molecular Formula	C19H19BF2N2O2
Molecular Weight	368.18 g/mol
Synonyms	BODIPY® 581/591 carboxylic acid; 4,4-Difluoro-5-(4-Methoxyphenyl)-4-bora-3a,4a-diaza-s-indacene-3-propionic Acid; BDP 581/591 acid; BODIPY FL-X, SE analogue; Boron Dipyrromethene 581/591 Carboxylic Acid; 3-(2,6-Dimethyl-4-bora-3a,4a-diaza-s-indacen-8-yl)propanoic Acid (derivative)
EINECS	Contact for details

Quality Control

Every batch of Bodipy 581/591 Carboxylic Acid is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure high purity and batch-to-batch consistency through HPLC, NMR, and mass spectrometry analysis. A detailed Certificate of Analysis (COA) documenting identity, purity, and performance characteristics is provided with each shipment to support your research and production quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. For long-term storage under inert atmosphere, consider sealing under argon or nitrogen. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Anhydrous basis)
Absorption (λmax)	~581 nm (in suitable solvent)
Emission (λmax)	~591 nm (in suitable solvent)
Extinction Coefficient (ε)	Contact for details
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.