Description

Cyanine 3 Bisethyl Dye Potassium Salt is a near-infrared fluorescent labeling reagent widely used in life science research and diagnostics. This compound is valued for its high molar absorptivity, excellent photostability, and water solubility, making it ideal for sensitive detection applications. It is primarily utilized by researchers and manufacturers in the fields of bioimaging, flow cytometry, nucleic acid detection, and immunoassay development.

Application

• Fluorescent Labeling of Biomolecules: Conjugation to antibodies, proteins, peptides, and oligonucleotides for detection and quantification.
• Live-Cell and Tissue Imaging: Used as a probe for confocal microscopy and in vivo imaging due to its near-infrared emission, which reduces background autofluorescence.
• Flow Cytometry: Employed for cell sorting and analysis by labeling specific cell surface markers or intracellular targets.
• Nucleic Acid Detection: Labeling of DNA and RNA probes for fluorescence in situ hybridization (FISH), PCR, and microarray applications.
• Diagnostic Assay Development: Key component in the development of fluorescent lateral flow assays and other point-of-care diagnostic tools.
• Drug Delivery Tracking: Conjugation to nanoparticles or therapeutic agents to monitor biodistribution and pharmacokinetics.

Basic Information

Product Name	Cyanine 3 Bisethyl Dye Potassium Salt
CAS No.	474972-41-3
Molecular Formula	C37H45KN2O6S2
Molecular Weight	717.00 g/mol (approx.)
Synonyms	Cy3 Bisethyl Dye Potassium Salt; Cy3 Bisethyl Potassium Salt; Sulfo-Cyanine3 Bisethyl Potassium Salt; 1,1'-(4,4,7,7-Tetramethyl-4,7-dihydro-1H-cyclopenta[b]pyrazine-2,3-diyl)bis(ethan-1-ylidene))bis(3-ethyl-2,3-dihydro-1,3-benzothiazol-3-ium) Potassium Salt; Cy3 SE Potassium Salt analog; Near-IR fluorescent dye potassium salt; Cyanine dye C37H45KN2O6S2
EINECS	Contact for details

Quality Control

Our Cyanine 3 Bisethyl Dye Potassium Salt is produced under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance/emission verification, and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Dark green to blue-green solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	548 - 552 nm
Emission Maximum	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.