Description

Cyanine 5 Monofunctional Hexanoic Acid Dye, Potassium Salt is a near-infrared fluorescent labeling reagent designed for the covalent conjugation of biomolecules. This water-soluble, monofunctional dye is essential for creating stable, bright fluorescent probes with minimal impact on the biological activity of the target molecule. It is a critical tool for researchers and manufacturers in the fields of bioconjugation, flow cytometry, in vivo imaging, and diagnostic assay development.

Application

• Bioconjugation and Probe Development: Covalent labeling of antibodies, peptides, proteins, and oligonucleotides for fluorescence-based assays.
• Flow Cytometry & Cell Sorting: Creating bright, photostable antibody-dye conjugates for high-resolution cell population analysis and sorting.
• In Vivo Imaging: Labeling targeting agents for non-invasive near-infrared fluorescence (NIRF) imaging in preclinical research.
• Diagnostic Assays: Development of lateral flow assays (LFAs), ELISA, and other immunoassays requiring stable fluorescent signals.
• Microscopy & Histology: Fluorescent staining of tissue sections and cellular structures for detailed imaging studies.
• Nucleic Acid Labeling: Tagging primers, probes, and other oligonucleotides for real-time PCR and fluorescence in situ hybridization (FISH).

Basic Information

Item	Detail
Product Name	Cyanine 5 Monofunctional Hexanoic Acid Dye, Potassium Salt
CAS No.	449175-58-0
Molecular Formula	C38H45KN2O6S2
Molecular Weight	728.99 g/mol
Synonyms	Cy5 Hexanoic Acid, Potassium Salt; Cy5 Mono NHS Ester (precursor form); Sulfo-Cyanine5 carboxylic acid, potassium salt; 1-(5-Carboxypentyl)-2-((1E,3E)-5-(1-(6-sulfonatohexyl)-3,3-dimethyl-5-sulfo-2,3-dihydro-1H-indol-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium, potassium salt; Cy5™ dye acid; Near-IR fluorescent label
EINECS	Contact for details

Quality Control

Every batch of our Cyanine 5 Monofunctional Hexanoic Acid Dye, Potassium Salt is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We provide a detailed Certificate of Analysis (COA) with each shipment, confirming identity, purity, and performance specifications. Our quality commitment ensures batch-to-batch consistency for reliable and reproducible experimental results.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	Dark blue to purple solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum (λem)	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Water Content (KF)	≤ 5.0 %
Free Amine (Spot Test)	Negative

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.